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A simple method for the production and preparation of hyaluronic acid (HA) is described. Three media were tested, which all supported relatively good yields of cells, but only in one of them were appreciable amounts of HA formed. In this medium, HA was produced during the logarithmic growth phase and showed a molecular weight of 7.3 x 10. HA prepared from 24-hr cultures of the same organism had a molecular weight of only 2.6 x 10. The kinetics of HA production in batch culture were studied, as well as of cell production and glucose consumption.
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