Cytokine responsiveness in cultured human small airway epithelial cells in relation to lung transplantation. 2005

Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
The First Department of Surgery, Tokyo Medical University, Tokyo, Japan.

BACKGROUND We report a new method for collecting and establishing small airway epithelial cells (SAEC). This method enables the evaluation of the cytokine responsiveness of SAEC, which is still unknown. In this study we evaluated intercellular adhesion molecule-1 (ICAM-1) expression on SAEC stimulated with several inflammatory cytokines and compared it with that on large airway epithelial cells (LAEC). METHODS LAEC and SAEC were treated with IFN-gamma, TNF-alpha, IL-1beta, or their combination. ICAM-1 expression under various conditions was quantified by flow cytometry. Furthermore, immunocytochemical staining was performed to determine intranuclear displacement of signal transducer and activator transcription 1 (Stat1) during ICAM-1 expression by various cytokine stimulations. RESULTS 1) ICAM-1 expression on both LAEC and SAEC was significantly increased by IFN-gamma stimulation alone and synergistically enhanced by IFN-gamma plus TNF-alpha or IL-1beta stimulation, 2) intranuclear displacement of Stat1 in SAEC by the stimulation with IFN-gamma plus TNF-alpha or IL-1beta was recognized earlier in comparison with that by IFN-gamma stimulation alone. CONCLUSIONS The previously unknown peripheral cytokine responsiveness and its mechanisms of SAEC were revealed by this study, which contributes to the understanding of chronic lung allograft rejection recognized around small airways.

UI MeSH Term Description Entries
D007371 Interferon-gamma The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES. Interferon Type II,Interferon, Immune,gamma-Interferon,Interferon, gamma,Type II Interferon,Immune Interferon,Interferon, Type II
D007375 Interleukin-1 A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation. IL-1,Lymphocyte-Activating Factor,Epidermal Cell Derived Thymocyte-Activating Factor,Interleukin I,Macrophage Cell Factor,T Helper Factor,Epidermal Cell Derived Thymocyte Activating Factor,Interleukin 1,Lymphocyte Activating Factor
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D001980 Bronchi The larger air passages of the lungs arising from the terminal bifurcation of the TRACHEA. They include the largest two primary bronchi which branch out into secondary bronchi, and tertiary bronchi which extend into BRONCHIOLES and PULMONARY ALVEOLI. Primary Bronchi,Primary Bronchus,Secondary Bronchi,Secondary Bronchus,Tertiary Bronchi,Tertiary Bronchus,Bronchi, Primary,Bronchi, Secondary,Bronchi, Tertiary,Bronchus,Bronchus, Primary,Bronchus, Secondary,Bronchus, Tertiary
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003584 Cytological Techniques Methods used to study CELLS. Cytologic Technics,Cytological Technic,Cytological Technics,Cytological Technique,Technic, Cytological,Technics, Cytological,Technique, Cytological,Techniques, Cytological,Cytologic Technic,Technic, Cytologic,Technics, Cytologic
D004847 Epithelial Cells Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells. Adenomatous Epithelial Cells,Columnar Glandular Epithelial Cells,Cuboidal Glandular Epithelial Cells,Glandular Epithelial Cells,Squamous Cells,Squamous Epithelial Cells,Transitional Epithelial Cells,Adenomatous Epithelial Cell,Cell, Adenomatous Epithelial,Cell, Epithelial,Cell, Glandular Epithelial,Cell, Squamous,Cell, Squamous Epithelial,Cell, Transitional Epithelial,Cells, Adenomatous Epithelial,Cells, Epithelial,Cells, Glandular Epithelial,Cells, Squamous,Cells, Squamous Epithelial,Cells, Transitional Epithelial,Epithelial Cell,Epithelial Cell, Adenomatous,Epithelial Cell, Glandular,Epithelial Cell, Squamous,Epithelial Cell, Transitional,Epithelial Cells, Adenomatous,Epithelial Cells, Glandular,Epithelial Cells, Squamous,Epithelial Cells, Transitional,Glandular Epithelial Cell,Squamous Cell,Squamous Epithelial Cell,Transitional Epithelial Cell
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006084 Graft Rejection An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient. Transplant Rejection,Rejection, Transplant,Transplantation Rejection,Graft Rejections,Rejection, Graft,Rejection, Transplantation,Rejections, Graft,Rejections, Transplant,Rejections, Transplantation,Transplant Rejections,Transplantation Rejections

Related Publications

Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
July 1984, Journal of applied physiology: respiratory, environmental and exercise physiology,
Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
July 2005, Respiratory research,
Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
October 1999, Blood,
Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
June 1995, Current eye research,
Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
April 1994, Cancer research,
Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
October 1988, Prostaglandins, leukotrienes, and essential fatty acids,
Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
August 2009, Journal of virology,
Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
October 1992, Biochimica et biophysica acta,
Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
January 2021, Frontiers in allergy,
Kuniharu Miyajima, and Shin Nakajima, and Masahiko Taguchi, and Aeru Hayashi, and Naohiro Kajiwara, and Osamu Uchida, and Takafumi Kono, and Mitsuru Takahashi, and Keiichi Kawanishi, and Mitsuru Tanaka, and Hajime Takizawa, and Harubumi Kato
January 2020, BioMed research international,
Copied contents to your clipboard!