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A first-derivative spectroscopic method for the simultaneous determination of bound and unbound drug in human serum and serum albumin solution was developed. As an example, the binding characteristics of rifampicin were studied. In serum albumin solution, the rifampicin bound and unbound fractions were determined at 473.5 and 475.8 nm, respectively. For human serum, the unbound fraction was determined at 479.4 nm. The results obtained by the first-derivative spectroscopic method were in agreement with those obtained by equilibrium dialysis. The proposed method is very simple and accurate when applied to measurements of drug:protein binding. Moreover, it allows a direct measurement of the bound and unbound forms without any physical separation.
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
December 1991,
Arzneimittel-Forschung,
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
July 1984,
Photochemistry and photobiology,
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
August 2008,
Colloids and surfaces. B, Biointerfaces,
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
December 2022,
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
January 2020,
Royal Society open science,
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
March 2012,
Journal of photochemistry and photobiology. B, Biology,
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
March 2017,
Journal of biomolecular structure & dynamics,
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
September 2007,
The journal of physical chemistry. B,
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
June 2014,
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica,
S Lachau, and
M A Rochas, and
A E Tufenkji, and
N Martin, and
P Levillain, and
G Houin
July 2009,
Guang pu xue yu guang pu fen xi = Guang pu,
