The pharmacological basis of sensory cortex-dependent learning and associated cortical reorganizations is only partially understood. In the Mongolian gerbil, the auditory cortex is critical for discriminating the directions of modulation of linearly frequency-modulated tones (FMs). To examine the role of N-methyl-D-aspartate (NMDA)-type glutamate receptors in FM discrimination learning, selective antagonists were used. Compared to vehicle-treated controls, both systemic administration of MK-801 before but not after training, and infusion of D-AP-5 into the auditory cortex after training caused retention deficits detectable 24h later. The amnesic actions were reversible and in a close temporal relation to memory formation. Acquisition performance and performance of an established FM discrimination reaction were not affected. These findings suggest that NMDA receptor activation is required for long-term memory consolidation in auditory cortex-dependent learning.