The present study has been done to evaluate the efficacy of vaccine during manufacturing, storage and distribution under fluctuation of temperature as the vaccines are very susceptible to temperature. The results showed that New castle disease virus induce infections in developing embryos that lead to death of embryo with few exceptions. Further infection is more rapidly fatal when the inoculated embryos are young, when the quantity of virus introduce is large and when the required incubation temperature is above 37 degrees C. Comparison of table 4 and 5, and graph 4 indicate that no significant difference was found in the viral content (HA) of local and imported vaccines before and after inoculation, when analyzed through ANOVA and 5% level of significance. Comparison of Table 6, 7 and 8 as well as Fig. 5 indicate that the end point of EID50 (local vaccine) decreases with the length of channel of distribution or we can say that these are inversely proportional to each other.