A real-time polymerase chain reaction assay for detection of Pneumocystis from bronchoalveolar lavage fluid. 2006

Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
Division of Clinical Microbiology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.

Pneumocystis jiroveci is an important cause of pneumonia in immunocompromised individuals. This organism cannot be cultured, and therefore, diagnosis relies on microscopic identification of the organism using stains or antibodies. Although simple, these tests are insensitive and require expertise for accurate interpretation. We developed a real-time polymerase chain reaction (PCR) assay that provides sensitive and objective detection of Pneumocystis from bronchoalveolar lavage fluid. Primers and fluorescence resonance energy transfer probes were developed that target the cdc2 gene of P. jiroveci. Assay sensitivity is 6 copies of target per microliter of sample. No cross-reactivity occurs with other pathogens, and the PCR assay has a 21% increase in clinical sensitivity as compared with Calcofluor white staining. The real-time PCR assay provides a sensitive, rapid, and objective method for the detection of Pneumocystis from bronchoalveolar lavage fluid.

UI MeSH Term Description Entries
D001992 Bronchoalveolar Lavage Fluid Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung. Alveolar Lavage Fluid,Bronchial Lavage Fluid,Lung Lavage Fluid,Bronchial Alveolar Lavage Fluid,Lavage Fluid, Bronchial,Lavage Fluid, Lung,Pulmonary Lavage Fluid,Alveolar Lavage Fluids,Bronchial Lavage Fluids,Bronchoalveolar Lavage Fluids,Lavage Fluid, Alveolar,Lavage Fluid, Bronchoalveolar,Lavage Fluid, Pulmonary,Lavage Fluids, Alveolar,Lavage Fluids, Bronchial,Lavage Fluids, Bronchoalveolar,Lavage Fluids, Lung,Lavage Fluids, Pulmonary,Lung Lavage Fluids,Pulmonary Lavage Fluids
D004271 DNA, Fungal Deoxyribonucleic acid that makes up the genetic material of fungi. Fungal DNA
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D005656 Fungal Proteins Proteins found in any species of fungus. Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001557 Benzenesulfonates Organic salts and esters of benzenesulfonic acid.
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013194 Staining and Labeling The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts. Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D045363 Pneumocystis carinii The prototype species of PNEUMOCYSTIS infecting the laboratory rat. It was formerly called Pneumocystis carinii f. sp. carinii. Strains that infect humans and cause PNEUMOCYSTIS PNEUMONIA were originally classified as Pneumocystis jirovecii or Pneumocystis carinii f. sp. hominis. Pneumocystis carinii f. sp. carinii,Pneumocystis carinii f. sp. hominis,Pneumocystis carinii f. sp. ratti,Pneumocystis jirovecii,Pneumocystis carini,Pneumocystis jiroveci

Related Publications

Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
September 2011, The American journal of the medical sciences,
Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
June 2012, Diagnostic microbiology and infectious disease,
Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
January 2010, Acta cytologica,
Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
January 2013, The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases,
Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
December 2017, The American journal of tropical medicine and hygiene,
Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
January 1995, Scandinavian journal of infectious diseases,
Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
February 1993, Journal of clinical microbiology,
Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
August 1996, Journal of clinical microbiology,
Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
January 2020, Canadian respiratory journal,
Rodney C Arcenas, and James R Uhl, and Seanne P Buckwalter, and Andrew H Limper, and Dana Crino, and Glenn D Roberts, and Nancy L Wengenack
November 2009, Diagnostic microbiology and infectious disease,
Copied contents to your clipboard!