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Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare can be present in the lungs of pigs at the same time. These three mycoplasma species all require similar growth conditions and can be recovered from clinical samples using the same media. We have developed a multiplex PCR as a helpful tool for rapid differentiation of these three species in the course of isolation. Based on the 16S ribosomal DNA sequences, three different forward primers and a single reverse primer were selected. Each forward primer was compared to available mycoplasma sequences, showing the primers to be specific. The three amplification products observed of 1129 bp (M. hyorhinis), 1000 bp (M. hyopneumoniae) and 754 bp (M. flocculare) were clearly distinguishable on a 1% agarose gel. In addition, no cross-reaction with Mycoplasma hyosynoviae, another porcine mycoplasma, was noted. This multiplex PCR using the proposed set of primers is the first reported assay that allows the simultaneous identification of the different Mycoplasma species isolated from the lungs of pigs.
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P Butaye, and
H Imberechts, and
J Peeters, and
A de Kruif, and
F Haesebrouck, and
D Maes
January 2017,
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F Haesebrouck, and
D Maes
June 1997,
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A de Kruif, and
F Haesebrouck, and
D Maes
January 2003,
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T Stakenborg, and
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H Imberechts, and
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A de Kruif, and
F Haesebrouck, and
D Maes
March 2003,
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T Stakenborg, and
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January 2004,
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D Maes
November 1997,
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A de Kruif, and
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D Maes
April 2008,
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T Stakenborg, and
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D Maes
February 2014,
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A de Kruif, and
F Haesebrouck, and
D Maes
February 1995,
PCR methods and applications,
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J Vicca, and
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A de Kruif, and
F Haesebrouck, and
D Maes
May 2008,
Wei sheng wu xue bao = Acta microbiologica Sinica,
