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Caramel Colour III is used as a colour additive in beers and a variety of foods. Beer is the most important single source of Caramel Colour III in the diet although consumption of dark beers has been decreasing in recent years. The Joint FAO/WHO Expert Committee on Food Additives (JECFA) has established an acceptable daily intake of 200 mg/kg/day for Caramel Colour III. The safety of Caramel Colour III has been questioned during recent years following feeding studies in the rat that were associated with reduced white cell and lymphocyte counts. These effects have been attributed to the presence of 2-acetyl-4(5)-tetrahydroxybutylimidazole (THI) in this class of caramel colour. Short-term oral toxicity studies were conducted on low-THI and high-THI samples of Caramel Colour III (13 wk) and on a sample of THI (28 days). In both studies, the test materials were mixed with demineralized water and the solutions were given to the animals ad lib. in the drinking fluid. In the 13-wk subchronic toxicity study of Caramel Colour III, groups of 20 rats/sex were given concentrations of caramel colour equivalent to intakes of 0, 10, 15 or 20 g low-THI caramel colour/kg body weight/day or 20 g/kg of a high-THI caramel colour. In the 4-wk toxicity study with THI, groups of 20 rats/sex were given 0, 8 or 64 ppm THI (equivalent to approx. 0, 0.9 or 7.2 mg/kg/day) and 10 rats/sex were given 1, 2, 4, 16 or 32 ppm THI (equivalent to approx. 0.1, 0.2, 0.5, 1.9 or 3.7 mg/kg/day) for 4 wk followed by a 2-wk recovery phase for 10 rats/sex in the 0, 8 and 64 ppm groups. Rats given Caramel Colour III had soft faeces; there were no other treatment-related clinical observations and no treatment-related deaths occurred. All treated groups given Caramel Colour III had lower food and fluid consumption than controls. Males given 15 or 20 g low-THI caramel colour/kg or 20 g high-THI caramel colour/kg and females given 20 g/kg of either type had lower body weights than controls. In the 4-wk toxicity study with THI, there were no treatment-related ante-mortem observations, and no effects on body weights or food consumption. Fluid consumption by males and females treated with 64 ppm THI was lower than that of controls.(ABSTRACT TRUNCATED AT 400 WORDS)
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
March 1992,
Toxicology and applied pharmacology,
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
May 1992,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
January 2015,
Food additives & contaminants. Part B, Surveillance,
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
January 1988,
Immunology and cell biology,
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
July 1986,
Journal of chromatography,
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
December 2013,
Preventive nutrition and food science,
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
October 1987,
Journal of chromatography,
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
June 1992,
Clinical and experimental immunology,
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
January 1989,
Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement,
K M MacKenzie, and
B G Boysen, and
W E Field, and
S R Petsel, and
C I Chappel, and
J L Emerson, and
J Stanley
May 1992,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
