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A rapid and sensitive method for detecting HIV-1 DNA sequences amplified by polymerase chain reaction (PCR) is described. The method uses solution phase hybridization for rapid and efficient annealing between digoxigenin-labeled targets and biotinylated capture probes. Hybrids containing biotin are captured onto streptavidin coated microwells and all other PCR components are washed away, including spurious amplification products. The presence of the digoxigenin-labeled amplified HIV target is then detected by anti-digoxigenin-alkaline phosphatase conjugates using the chemiluminescent substrate PPD. This approach maintains high specificity by nucleic acid dependent capture, and high sensitivity by efficient solution hybridization. The method is rapid (2 hours), and capable of detecting 10 HIV targets.
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
August 1996,
Nucleic acids research,
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
May 1991,
Journal of virological methods,
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
February 1993,
Analytical biochemistry,
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
December 1994,
Der Unfallchirurg,
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
January 1994,
Acta veterinaria Hungarica,
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
June 1992,
Diagnostic molecular pathology : the American journal of surgical pathology, part B,
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
January 1989,
BioTechniques,
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
November 1990,
AIDS research and human retroviruses,
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
October 1994,
BioTechniques,
K Suzuki, and
N Okamoto, and
S Watanabe, and
T Kano
June 1990,
AIDS research and human retroviruses,
