BIOMAT Database Logo BIOMAT Database Logo

Inhibition of multiple gene expression and virus replication of HBV by stable RNA interference in 2.2.15 cells. 2006

Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
State Key Laboratory of Biocontrol and Department of Biochemistry, College of Life Sciences, Sun Yat-sen Zhongshan University, Guangzhou 510275, People's Republic of China [corrected]

OBJECTIVE Hepatitis B virus (HBV) infection is a world-wide health problem. Recent studies have demonstrated the efficacy of RNA interference (RNAi) against HBV replication at cell culture and animal levels using transient transfection. The present study was to determine whether the stable transfection of short hairpin RNA (shRNA)-producing vector could achieve potent and sustained inhibition of the HBV replication in 2.2.15 cells. METHODS shRNA-producing vector against HBV and the empty vector were stably transfected into the 2.2.15 cells respectively. A series of experiments were performed in the producing stable lines to determine the changes of viral protein expression and replication. RESULTS The HBV protein expression and viral replication were suppressed dramatically and stably by the integrated shRNA-producing vectors. Most importantly, this suppression effect persists after 30 passages. CONCLUSIONS Our data provided the possibility of continuous and stable inhibition of HBV protein expression and replication in patients using RNAi, suggesting a potential clinical application of this novel approach. Furthermore, the established stable transfected cell lines provided a good platform for understanding the mechanism of anti-HBV by RNAi.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008113 Liver Neoplasms Tumors or cancer of the LIVER. Cancer of Liver,Hepatic Cancer,Liver Cancer,Cancer of the Liver,Cancer, Hepatocellular,Hepatic Neoplasms,Hepatocellular Cancer,Neoplasms, Hepatic,Neoplasms, Liver,Cancer, Hepatic,Cancer, Liver,Cancers, Hepatic,Cancers, Hepatocellular,Cancers, Liver,Hepatic Cancers,Hepatic Neoplasm,Hepatocellular Cancers,Liver Cancers,Liver Neoplasm,Neoplasm, Hepatic,Neoplasm, Liver
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D006513 Hepatitis B e Antigens A closely related group of antigens found in the plasma only during the infective phase of hepatitis B or in virulent chronic hepatitis B, probably indicating active virus replication; there are three subtypes which may exist in a complex with immunoglobulins G. HBeAg,Hepatitis B e Antigen,Hepatitis Be Antigen,e Antigen,e Antigens,HBe Ag-1,HBe Ag-2,Hepatitis Be Antigens,Antigen, Hepatitis Be,Antigen, e,Antigens, Hepatitis Be,Antigens, e,Be Antigen, Hepatitis,Be Antigens, Hepatitis
D006514 Hepatitis B Surface Antigens Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen. Australia Antigen,HBsAg,Hepatitis B Surface Antigen,Antigen, Australia
D006515 Hepatitis B virus The type species of the genus ORTHOHEPADNAVIRUS which causes human HEPATITIS B and is also apparently a causal agent in human HEPATOCELLULAR CARCINOMA. The Dane particle is an intact hepatitis virion, named after its discoverer. Non-infectious spherical and tubular particles are also seen in the serum. Dane Particle,Hepatitis Virus, Homologous Serum,B virus, Hepatitis,Hepatitis B viruses,Particle, Dane,viruses, Hepatitis B
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA

Related Publications

Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
[Inhibition of HBV DNA replication and expression in 2.2.15 hepatoma cells infected with AFP-mediated HBX antisense RNA].
May 2003, Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology,
Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
[Inhibition of HBV replication and antigen expression by RNA interference against different targets].
September 2006, Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology,
Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
Stable inhibition of hepatitis B virus expression and replication in HepG2.2.15 cells by RNA interference based on retrovirus delivery.
January 2007, Journal of biotechnology,
Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
Inhibition of HBV-DNA replication and expression by siRNA based on magnetic nanoparticles transfering in HepG2 2.2.15 cells.
June 2010, Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences,
Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
Inhibition of human La protein by RNA interference downregulates hepatitis B virus mRNA in 2.2.15 cells.
July 2004, World journal of gastroenterology,
Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
Inhibition of hepatitis B virus expression and replication by RNA interference.
April 2003, Hepatology (Baltimore, Md.),
Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
Inhibition of virus replication by RNA interference.
January 2003, Journal of biomedical science,
Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
Inhibition of hepatitis B virus replication by shRNAs in stably HBV expressed HEPG2 2.2.15 cell lines.
January 2007, Archives of virology,
Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
Inhibition of viral gene expression and replication in mosquito cells by dsRNA-triggered RNA interference.
August 2002, Molecular therapy : the journal of the American Society of Gene Therapy,
Xiangrong Ren, and Guangbin Luo, and Zhenhui Xie, and Lingjun Zhou, and Xiangping Kong, and Anlong Xu
Inhibition of monkeypox virus replication by RNA interference.
November 2009, Virology journal,
Copied contents to your clipboard!