BIOMAT Database Logo BIOMAT Database Logo

Hypothermia-enhanced splenic cytokine gene expression is independent of the sympathetic nervous system. 2006

Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506, USA.

Splenic nerve denervation abrogates enhanced splenic cytokine gene expression responses to acute heating, demonstrating that hyperthermia-induced activation of splenic sympathetic nerve discharge (SND) increases splenic cytokine gene expression. Hypothermia alters SND responses; however, the role of the sympathetic nervous system in mediating splenic cytokine gene expression responses to hypothermia is not known. The purpose of the present study was to determine the effect of hypothermia on the relationship between the sympathetic nervous system and splenic cytokine gene expression in anesthetized F344 rats. Gene expression analysis was performed using a microarray containing 112 genes, representing inflammatory cytokines, chemokines, cytokine/chemokine receptors and housekeeping genes. A subset of differentially expressed genes was verified by real-time RT-PCR analysis. Splenic SND was decreased significantly during cooling (core temperature decreased from 38 to 30 degrees C) in splenic-intact rats but remained unchanged in sham-cooled splenic-intact rats (core temperature maintained at 38 degrees C). Hypothermia upregulated the transcripts of several genes, including, chemokine ligands CCL2, CXCL2, CXCL10, and CCL20, and interleukins IL-1alpha, IL-1beta, and IL-6. Gene expression responses to hypothermia were similar for the majority of cytokine genes in splenic-intact and splenic-denervated rats. These results suggest that hypothermia-enhanced splenic cytokine gene expression is independent of splenic SND.

UI MeSH Term Description Entries
D007035 Hypothermia Lower than normal body temperature, especially in warm-blooded animals. Hypothermia, Accidental,Accidental Hypothermia,Accidental Hypothermias,Hypothermias,Hypothermias, Accidental
D011916 Rats, Inbred F344 An inbred strain of rat that is used for general BIOMEDICAL RESEARCH purposes. Fischer Rats,Rats, Inbred CDF,Rats, Inbred Fischer 344,Rats, F344,Rats, Inbred Fisher 344,CDF Rat, Inbred,CDF Rats, Inbred,F344 Rat,F344 Rat, Inbred,F344 Rats,F344 Rats, Inbred,Inbred CDF Rat,Inbred CDF Rats,Inbred F344 Rat,Inbred F344 Rats,Rat, F344,Rat, Inbred CDF,Rat, Inbred F344,Rats, Fischer
D001794 Blood Pressure PRESSURE of the BLOOD on the ARTERIES and other BLOOD VESSELS. Systolic Pressure,Diastolic Pressure,Pulse Pressure,Pressure, Blood,Pressure, Diastolic,Pressure, Pulse,Pressure, Systolic,Pressures, Systolic
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006339 Heart Rate The number of times the HEART VENTRICLES contract per unit of time, usually per minute. Cardiac Rate,Chronotropism, Cardiac,Heart Rate Control,Heartbeat,Pulse Rate,Cardiac Chronotropy,Cardiac Chronotropism,Cardiac Rates,Chronotropy, Cardiac,Control, Heart Rate,Heart Rates,Heartbeats,Pulse Rates,Rate Control, Heart,Rate, Cardiac,Rate, Heart,Rate, Pulse
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013154 Spleen An encapsulated lymphatic organ through which venous blood filters.
D013564 Sympathetic Nervous System The thoracolumbar division of the autonomic nervous system. Sympathetic preganglionic fibers originate in neurons of the intermediolateral column of the spinal cord and project to the paravertebral and prevertebral ganglia, which in turn project to target organs. The sympathetic nervous system mediates the body's response to stressful situations, i.e., the fight or flight reactions. It often acts reciprocally to the parasympathetic system. Nervous System, Sympathetic,Nervous Systems, Sympathetic,Sympathetic Nervous Systems,System, Sympathetic Nervous,Systems, Sympathetic Nervous
D016207 Cytokines Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner. Cytokine
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Sympathetic nervous system activity during hypothermia.
October 1984, Critical care medicine,
Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Cytokine gene expression within the central nervous system.
December 1998, Cellular and molecular neurobiology,
Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Sympathetic nervous system "switch off" with severe hypothermia.
September 1983, Critical care medicine,
Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Central nervous system cytokine gene expression: modulation by lead.
January 2011, Journal of biochemical and molecular toxicology,
Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Influence of the sympathetic nervous system on renal function during hypothermia.
July 1998, Acta physiologica Scandinavica,
Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Developmental gene expression of sympathetic nervous system tumors reflects their histogenesis.
January 1998, Laboratory investigation; a journal of technical methods and pathology,
Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Marek's disease virus-induced transient paralysis is associated with cytokine gene expression in the nervous system.
January 2006, Viral immunology,
Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Cytokine expression in the lesioned nervous system.
April 1994, Neuropathology and applied neurobiology,
Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Target-independent cholinergic differentiation in the rat sympathetic nervous system.
April 1997, Proceedings of the National Academy of Sciences of the United States of America,
Chanran K Ganta, and Bryan G Helwig, and Frank Blecha, and Roman R Ganta, and Richard Cober, and Sujatha Parimi, and Timothy I Musch, and Richard J Fels, and Michael J Kenney
Conditioned suppression of contact sensitivity is independent of sympathetic splenic innervation.
October 2000, American journal of physiology. Regulatory, integrative and comparative physiology,
Copied contents to your clipboard!