BIOMAT Database Logo BIOMAT Database Logo

[Superoxide production of polymorphonuclear leukocytes in endotoxin shock and antioxidative effect of coenzyme Q10]. 1991

H Suzuki
First Department of Surgery, Nippon Medical School, Tokyo, Japan.

The present study was designed to determine the superoxide production of polymorphonuclear leukocytes (PMN) in endotoxin shock and the antioxidative effect of coenzyme Q10. PMN were collected from rats before and after the intravenous administration of endotoxin. PMN were also collected from healthy humans. Superoxide production of PMN was measured by the cytochrome C method. Lipid peroxide in the liver was examined by the TBA method. Twelve hours after the intravenous administration of 5 mg/kg endotoxin, superoxide production of PMN was 13 times higher than that in the control rats, and the amount of lipid peroxide in the liver was increased. In coenzyme Q10 treated endotoxin shock rats, superoxide production was significantly decreased, and lipid peroxide production in the liver was also inhibited. These findings suggest that endotoxin has a priming effect on the superoxide production of PMN and induces lipid peroxidation. Furthermore coenzyme Q10 has an anti-endotoxin shock effect by inhibiting the superoxide production of PMN and lipid peroxidation in the liver.

UI MeSH Term Description Entries
D008054 Lipid Peroxides Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension. Fatty Acid Hydroperoxide,Lipid Peroxide,Lipoperoxide,Fatty Acid Hydroperoxides,Lipid Hydroperoxide,Lipoperoxides,Acid Hydroperoxide, Fatty,Acid Hydroperoxides, Fatty,Hydroperoxide, Fatty Acid,Hydroperoxide, Lipid,Hydroperoxides, Fatty Acid,Peroxide, Lipid,Peroxides, Lipid
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D009504 Neutrophils Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes. LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D003067 Coenzymes Small molecules that are required for the catalytic function of ENZYMES. Many VITAMINS are coenzymes. Coenzyme,Enzyme Cofactor,Cofactors, Enzyme,Enzyme Cofactors,Cofactor, Enzyme
D005609 Free Radicals Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. Free radicals include reactive oxygen and nitrogen species (RONS). They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated. Free Radical
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012772 Shock, Septic Sepsis associated with HYPOTENSION or hypoperfusion despite adequate fluid resuscitation. Perfusion abnormalities may include but are not limited to LACTIC ACIDOSIS; OLIGURIA; or acute alteration in mental status. Endotoxin Shock,Septic Shock,Shock, Endotoxic,Shock, Toxic,Toxic Shock,Toxic Shock Syndrome,Endotoxin Shocks,Shock Syndrome, Toxic,Shock, Endotoxin,Shocks, Endotoxin,Toxic Shock Syndromes
D013481 Superoxides Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides. Superoxide Radical,Superoxide,Superoxide Anion

Related Publications

H Suzuki
Effect of coenzyme Q10 on endotoxin shock in dogs.
June 1986, Critical care medicine,
H Suzuki
[Effect of coenzyme Q10 on endotoxin induced hepatocyte injury modulation of endotoxin-activated polymorphonuclear neutrophils].
June 1995, Nihon Ika Daigaku zasshi,
H Suzuki
Stimulus-specific effects of endotoxin on superoxide production by rabbit polymorphonuclear leukocytes.
January 1987, The Yale journal of biology and medicine,
H Suzuki
Effect of coenzyme Q10 on superoxide production in rats with reperfusion injuries.
March 1999, Scandinavian journal of plastic and reconstructive surgery and hand surgery,
H Suzuki
Fluoride-induced superoxide production in rabbit polymorphonuclear leukocytes.
July 1981, Biochemical pharmacology,
H Suzuki
Involvement of superoxide generated by polymorphonuclear leukocytes in endotoxin-induced uveitis.
April 2000, Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
H Suzuki
Effect of microtubule-disrupting agents on superoxide production in human polymorphonuclear leukocytes.
December 1982, Biochimica et biophysica acta,
H Suzuki
Antioxidative effect of dietary coenzyme Q10 in human blood plasma.
January 1994, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition,
H Suzuki
Regulation of superoxide production in human polymorphonuclear leukocytes (PMNs).
May 1997, Biochemical Society transactions,
H Suzuki
Superoxide production by polymorphonuclear leukocytes. A cytochemical approach.
January 1986, Histochemistry,
Copied contents to your clipboard!