-transparent.png){kind=logo}
Nitric oxide (NO) mediates several biological actions, including relaxation of blood vessels, cytotoxicity of activated macrophages, and formation of cGMP by activation of glutamate receptors in cerebellar slices. Nitric oxide synthase (EC 1.14.23.-) immunoreactivity is colocalized with nicotinamide adenine di-nucleotide phosphate diaphorase in neurons that are uniquely resistant to toxic insults. We show that the nitric oxide synthase inhibitors, N omega-nitro-L-arginine (EC50 = 20 microM) and N omega-monomethyl-L-arginine (EC50 = 170 microM), prevent neurotoxicity elicited by N-methyl-D-aspartate and related excitatory amino acids. This effect is competitively reversed by L-arginine. Depletion of the culture medium of arginine by arginase or arginine-free growth medium completely attenuates N-methyl-D-aspartate toxicity. Sodium nitroprusside, which spontaneously releases NO, produces dose-dependent cell death that parallels cGMP formation. Hemoglobin, which complexes NO, prevents neurotoxic effects of both N-methyl-D-aspartate and sodium nitroprusside. These data establish that NO mediates the neurotoxicity of glutamate.
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
July 2000,
Journal of neurochemistry,
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
May 1999,
Chinese medical journal,
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
January 1999,
Brain research,
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
May 2004,
Planta medica,
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
March 2006,
European journal of pharmacology,
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
October 2021,
World journal of psychiatry,
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
October 1998,
Nihon yakurigaku zasshi. Folia pharmacologica Japonica,
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
January 2017,
Clinica e investigacion en arteriosclerosis : publicacion oficial de la Sociedad Espanola de Arteriosclerosis,
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
August 1992,
Neuroscience letters,
V L Dawson, and
T M Dawson, and
E D London, and
D S Bredt, and
S H Snyder
June 1996,
Neurodegeneration : a journal for neurodegenerative disorders, neuroprotection, and neuroregeneration,
