Adrenergic transmitters have been implicated in the regulation of male sexual behavior. In the present study the contribution of alpha 2-adrenoceptors, located within the central nervous system, was evaluated. Sexually experienced male Long-Evans rats were implanted with a permanent cannula in either the third cerebral ventricle or, unilaterally, in the medial preoptic area. Baseline mating tests were administered 4, 7 and 10 days after surgery and only males ejaculating (at least) in the 7 and 10 day tests were used. Clonidine-evoked dose-dependent decrements in the number of males mounting, intromitting and ejaculating, with administration into the medial preoptic area more effective than into the 3rd ventricle. In those animals mating, administration of 20 nmol into the 3rd ventricle was associated with decreases in the latency to ejaculation and intercopulatory interval, whereas administration of 2 nmol into the medial preoptic area was associated with increases in these parameters and decreases in the ejaculatory threshold. Administration of yohimbine into the medial preoptic area attenuated the effects of systemically-administered clonidine and the systemic administration of yohimbine completely prevented the copulatory suppression induced by administration of clonidine into the medial preoptic area. It is suggested that central alpha 2-adrenoceptors are important in the control of male sexual behavior and that alterations in adrenergic mechanisms in the medial preoptic area may underlie sexual dysfunction of various etiologies.