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Another publication (rand, R. P., Pangborn, W., Purdon, A. D., and Tinker, D. O.(1975) Can. j. Biochem. 53, 189-195) has established that lysolecithin and cholesterol interact to form an equimolar complex. We have investigated this complex using the techniques of electron spin resonance (e.s.r) and electronmicroscopy. By varying the cholesterol concentration with lysolecithin in both thin films and dispersions studied by these techniques, we have observed the interaction between lysolecithin and equimolar complex below 50 mol % cholesterol, and between crystalline cholesterol and equimolar complex above 50 mol % cholesterol. We have observed an interesting alteration in morphology by electron microscopy, and an isotropic to anisotropic spectral change using 3-dosylcholestane and 12-doxylstearic acid spin-labelled probes when the cholesterol concentration is increased from 20 to 33 mol %. The equimolar complex is stable in the presence of crystalline cholesterol, and exhibits no phase changes in the physiological temperature range. Implications for membrane structure are discussed.
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
December 1979,
Chemico-biological interactions,
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
July 1974,
Journal of biochemistry,
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
January 1983,
FEBS letters,
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
August 1996,
Biophysical journal,
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
February 1997,
Biophysical journal,
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
April 1972,
Analytical chemistry,
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
April 1963,
British medical journal,
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
June 1990,
Analytical chemistry,
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
September 1985,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
A D Purdon, and
J C Hsia, and
L Pinteric, and
D O Tinker, and
R P Rand
January 1981,
Molecular biology, biochemistry, and biophysics,
