-transparent.png){kind=logo}
Kinetics of hydrolysis of acetylthiocholine (ATCH) and acetylcholine (ACH) by butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE) are studied. ATCH is used for testing of enzymatic hydrolysis of ACH in vitro, because mechanism of ATCH hydrolysis is qualitatively similar to ACH and its reaction course can be quantitatively on-line measured by two independent methods: spectrophotometrical (determination of thiocholine - product of ATCH hydrolysis - using Ellman's method) and electrochemical (determination of acetic acid - product of ATCH hydrolysis - by pH-stat method). All tested hydrolyses correspond to the Michaelis-Menten's equation with the second irreversible step up to the total exhaustion of the substrate. The correlations were made by means of differential and integral kinetic equations describing Michaelis-Menten model. The optimal values of Michaelis constant (KM), maximum velocity (Vm), kinetic constants of single reaction steps and absolute concentration of the used enzyme were calculated for each experiment.
A Komersová, and
K Komers, and
P Zdrazilová
January 1974,
Ukrains'kyi biokhimichnyi zhurnal,
A Komersová, and
K Komers, and
P Zdrazilová
January 2002,
Zeitschrift fur Naturforschung. C, Journal of biosciences,
A Komersová, and
K Komers, and
P Zdrazilová
June 1952,
Archives of biochemistry and biophysics,
A Komersová, and
K Komers, and
P Zdrazilová
September 1984,
The Journal of biological chemistry,
A Komersová, and
K Komers, and
P Zdrazilová
October 2006,
Toxicology letters,
A Komersová, and
K Komers, and
P Zdrazilová
January 1953,
Naunyn-Schmiedebergs Archiv fur experimentelle Pathologie und Pharmakologie,
A Komersová, and
K Komers, and
P Zdrazilová
May 1972,
Molecular pharmacology,
A Komersová, and
K Komers, and
P Zdrazilová
June 1982,
Indian journal of experimental biology,
A Komersová, and
K Komers, and
P Zdrazilová
January 1968,
Histochemie. Histochemistry. Histochimie,
A Komersová, and
K Komers, and
P Zdrazilová
February 1955,
Produits pharmaceutiques,
