Immune and trophoblast cells at the rhesus monkey maternal-fetal interface. 2006

Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
National Primate Research Center and Department of Obstetrics and Gynecology, University of Wisconsin-Madison, USA.

To promote the use of the nonhuman primate model for the study of the cellular and molecular biology of maternal-fetal interactions and placental development during early pregnancy, we have developed protocols for the isolation and characterization of placental trophoblasts and decidual immune cells from the rhesus monkey. In this chapter, we provide protocols for trophoblast and decidual immune cell isolation, phenotyping of isolated cells by flow cytometry, and analysis of placental and decidual tissues by immunohistochemistry. Information on antibodies for these analyses are also provided, which is an important consideration when attempting to use anti-human antibodies for the study of nonhuman primates.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008253 Macaca mulatta A species of the genus MACACA inhabiting India, China, and other parts of Asia. The species is used extensively in biomedical research and adapts very well to living with humans. Chinese Rhesus Macaques,Macaca mulatta lasiota,Monkey, Rhesus,Rhesus Monkey,Rhesus Macaque,Chinese Rhesus Macaque,Macaca mulatta lasiotas,Macaque, Rhesus,Rhesus Macaque, Chinese,Rhesus Macaques,Rhesus Macaques, Chinese,Rhesus Monkeys
D010920 Placenta A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES). Placentoma, Normal,Placentome,Placentas,Placentomes
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D014327 Trophoblasts Cells lining the outside of the BLASTOCYST. After binding to the ENDOMETRIUM, trophoblasts develop into two distinct layers, an inner layer of mononuclear cytotrophoblasts and an outer layer of continuous multinuclear cytoplasm, the syncytiotrophoblasts, which form the early fetal-maternal interface (PLACENTA). Cytotrophoblasts,Syncytiotrophoblasts,Trophoblast,Cytotrophoblast,Syncytiotrophoblast

Related Publications

Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
January 1992, Developmental and comparative immunology,
Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
January 2004, Placenta,
Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
January 2005, Reproductive biology and endocrinology : RB&E,
Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
February 2013, Cell death & disease,
Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
January 2003, Science (New York, N.Y.),
Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
December 2005, Journal of reproductive immunology,
Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
January 2019, Science immunology,
Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
October 2020, Journal of cellular and molecular medicine,
Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
July 2020, Reproduction (Cambridge, England),
Thaddeus G Golos, and Gennadiy I Bondarenko, and Edith E Breburda, and Svetlana V Dambaeva, and Maureen Durning, and Igor I Slukvin
January 1992, American journal of reproductive immunology (New York, N.Y. : 1989),
Copied contents to your clipboard!