BIOMAT Database Logo BIOMAT Database Logo

Glucosyltransferase from Streptococcus sobrinus Catalyzes Glucosylation of Catechin. 1995

K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada

We previously showed that the polymeric forms of polyphenols present in oolong tea extract exhibited strong inhibitory activities against glucosyltransferases (GTases) of mutans streptococci, while green tea extract, which is rich in catechins, did not show such GTase-inhibitory activities. In this study, (+)-catechin [2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-3,5,7-triol] was found to be glucosylated by the GTase of Streptococcus sobrinus 6715 with sucrose as the substrate, and the product was identified as 4(prm1)-O-(alpha)-d-glucopyranosyl-(+)-catechin, with a molecular weight of 452. The (alpha)-glucosylated (+)-catechin did not exhibit significant inhibition of glucan synthesis from sucrose by the GTase, which is in contrast to the polymeric polyphenols isolated from oolong tea leaves.

UI MeSH Term Description Entries

Related Publications

K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
Peroxidase-catalyzed generation of catechin oligomers that inhibit glucosyltransferase from Streptococcus sobrinus.
September 1996, FEMS microbiology letters,
K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
Purification of a fourth glucosyltransferase from Streptococcus sobrinus.
November 1989, Journal of bacteriology,
K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28.
September 1987, Journal of bacteriology,
K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
Oral immunization of humans with Streptococcus sobrinus glucosyltransferase.
November 1987, Infection and immunity,
K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
Homology of glucosyltransferase gene and protein sequences from Streptococcus sobrinus and Streptococcus mutans.
March 1988, Journal of dental research,
K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
Effect of magnesium ions on secretion of glucosyltransferase from Streptococcus sobrinus.
January 1989, Microbios,
K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
Nigerooligosaccharide acceptor reaction of Streptococcus sobrinus glucosyltransferase GTF-I.
June 2000, Carbohydrate research,
K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
Single-molecule imaging of interaction between dextran and glucosyltransferase from Streptococcus sobrinus.
February 2000, Journal of bacteriology,
K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
Characterization of glucosyltransferase expressed from a Streptococcus sobrinus gene cloned in Escherichia coli.
April 1987, Journal of general microbiology,
K Nakahara, and M Kontani, and H Ono, and T Kodama, and T Tanaka, and T Ooshima, and S Hamada
DNA sequence of the glucosyltransferase gene of serotype d Streptococcus sobrinus.
January 1993, DNA sequence : the journal of DNA sequencing and mapping,
Copied contents to your clipboard!