BIOMAT Database Logo BIOMAT Database Logo

A comparative analysis of the putative regulatory regions in human genes for the alpha-subunit family of Na(+)-K+ ATPase. 1991

I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow.

The sequences of a 1.5 kb long stretch of the 5' flanking region of the gene for the alpha 3 isoform of the catalytic subunit of human Na(+)-K+ ATPase (located on chromosome 19) and of more than a 2 kb stretch of the 5' flanking region of the gene for the alpha 2 isoform (located on chromosome 1) have been determined. Transcription start sites for the gene for the alpha 3 isoform have been mapped at positions -152 and -155 relative to the translation initiation codon by primer extension analysis and S1-nuclease mapping of mRNA from human brain. The 5' flanking region of the gene for isoform alpha 3 contains a CCAAT box on the noncoding chain and six putative Sp1 binding sites. Absence of a conventional TATA box and a high GC content are other features of the region. The 5' upstream region of the gene for the alpha 2 isoform contains potential TATA and CCAAT boxes and one potential Sp1 binding site. Upstream of the putative TATA box there is an octanucleotide repeat, GGGGGAGA, which is also found in several eukaryotic genes in analogous positions. Pairwise comparison of the putative 5' regulatory regions of the genes coding for the different isoforms of the Na(+)-K(+)-ATPase catalytic subunit shows the existence of conserved elements, as well as of oligonucleotide blocks with very different structures. It is suggested that the differences in the primary structure of the 5' upstream regions may provide the basis for tissue-specific expression of the Na(+)-K(+)-ATPase isoforms.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D012045 Regulatory Sequences, Nucleic Acid Nucleic acid sequences involved in regulating the expression of genes. Nucleic Acid Regulatory Sequences,Regulatory Regions, Nucleic Acid (Genetics),Region, Regulatory,Regions, Regulatory,Regulator Regions, Nucleic Acid,Regulatory Region,Regulatory Regions
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000254 Sodium-Potassium-Exchanging ATPase An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients. ATPase, Sodium, Potassium,Adenosinetriphosphatase, Sodium, Potassium,Na(+)-K(+)-Exchanging ATPase,Na(+)-K(+)-Transporting ATPase,Potassium Pump,Sodium Pump,Sodium, Potassium ATPase,Sodium, Potassium Adenosinetriphosphatase,Sodium-Potassium Pump,Adenosine Triphosphatase, Sodium, Potassium,Na(+) K(+)-Transporting ATPase,Sodium, Potassium Adenosine Triphosphatase,ATPase Sodium, Potassium,ATPase, Sodium-Potassium-Exchanging,Adenosinetriphosphatase Sodium, Potassium,Pump, Potassium,Pump, Sodium,Pump, Sodium-Potassium,Sodium Potassium Exchanging ATPase,Sodium Potassium Pump
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D001665 Binding Sites The parts of a macromolecule that directly participate in its specific combination with another molecule. Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D012689 Sequence Homology, Nucleic Acid The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function. Base Sequence Homology,Homologous Sequences, Nucleic Acid,Homologs, Nucleic Acid Sequence,Homology, Base Sequence,Homology, Nucleic Acid Sequence,Nucleic Acid Sequence Homologs,Nucleic Acid Sequence Homology,Sequence Homology, Base,Base Sequence Homologies,Homologies, Base Sequence,Sequence Homologies, Base

Related Publications

I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
[Human Na+,K+-ATPase genes. Gene family and pseudogene for beta-subunit].
January 1990, Doklady Akademii nauk SSSR,
I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
The family of human Na+,K+-ATPase genes. A partial nucleotide sequence related to the alpha-subunit.
March 1987, FEBS letters,
I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
Chromosomal localization of human Na+, K+-ATPase alpha- and beta-subunit genes.
February 1988, Genomics,
I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
[The family of human Na+,K+-ATPase genes. At least 5 genes and/or pseudogenes homologous to alpha-subunit].
January 1987, Doklady Akademii nauk SSSR,
I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
Immunochemical identification of exposed regions of the Na+,K(+)-ATPase alpha-subunit.
July 1993, FEBS letters,
I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
A putative fourth Na+,K(+)-ATPase alpha-subunit gene is expressed in testis.
December 1994, Proceedings of the National Academy of Sciences of the United States of America,
I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
Assembly of the chimeric Na+/K+-ATPase and H+/K+-ATPase beta-subunit with the Na+/K+-ATPase alpha-subunit.
December 1997, Biochimica et biophysica acta,
I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
Multiple genes encode the human Na+,K+-ATPase catalytic subunit.
June 1987, Proceedings of the National Academy of Sciences of the United States of America,
I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
Assembly of Na,K-ATPase alpha-subunit isoforms with Na,K-ATPase beta-subunit isoforms and H,K-ATPase beta-subunit.
July 1994, The Journal of biological chemistry,
I V Malyshev, and D A Bessarab, and Orlova MYu, and K E Petrukhin, and S V Volik, and N E Broude, and G S Monastyrskaya, and N N Modyanov, and E D Sverdlov
Characterization of two genes for the human Na,K-ATPase beta subunit.
October 1989, Genomics,
Copied contents to your clipboard!