-transparent.png){kind=logo}
Doxycycline (Dc) has been demonstrated to inhibit cell growth and induce apoptosis in tumor cells, although its mechanism of action is not fully understood. The present study demonstrates that apoptosis can be induced in HeLa cells. Western blot data demonstrated that cytochrome c (Cyt c), Smac (the second mitochondria-derived activator of caspase), calpain I, caspase-9, -3 and -8 were involved in the apoptotic process, while the pan caspase inhibitor zVAD-fmk almost completely inhibited Dc-induced apoptosis. We further demonstrated that the release of mitochondrial proteins and the activation of calpains occurred upstream of the caspase cascade, in which caspase-9 was activated in response to the release of Cyt c, that caspase-8 activation was caspase and calpain dependent, and that caspase-3 was activated mainly by caspase-8 and -9. Caspase-8 played important roles in the activation of caspase-3 and induction of apoptosis, whereas the role of the caspase-9 was limited.
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
April 2006,
Apoptosis : an international journal on programmed cell death,
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
January 2014,
Cytotechnology,
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
February 2007,
International journal of cancer,
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
October 2012,
Cardiovascular research,
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
August 2006,
Biochemical and biophysical research communications,
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
January 2013,
PloS one,
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
July 2015,
Biological research,
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
May 2020,
Molecular biology reports,
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
October 2019,
Journal of cellular biochemistry,
J Wu, and
T Liu, and
J Xie, and
F Xin, and
L Guo
December 2000,
The Journal of biological chemistry,
