BIOMAT Database Logo BIOMAT Database Logo

Improvement of Bacillus thuringiensis delta-endotoxins synthesis yields through acquisition of erythromycin resistance. 2006

Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, Tunisia P.O. Box K, 3038, Sfax, Tunisia.

The acquisition of the erythromycin resistance by Bacillus thuringiensis kurstaki improved yields of delta-endotoxins in sporulating cells ranging from 134 to 215%. Resistance to erythromycin decreased the final spore count by at least 50%. Consequently, erythromycin resistance is an efficient tool for the improvement of bioinsecticides yields with a high ratio of delta-endotoxins to spores.

UI MeSH Term Description Entries
D011498 Protein Precursors Precursors, Protein
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D004731 Endotoxins Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells. Endotoxin
D004917 Erythromycin A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins. Erycette,Erymax,Erythromycin A,Erythromycin C,Erythromycin Lactate,Erythromycin Phosphate,Ilotycin,T-Stat,Lactate, Erythromycin,Phosphate, Erythromycin,T Stat,TStat
D006460 Hemolysin Proteins Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS. Hemolysin,Hemolysins,Hemalysins,Proteins, Hemolysin
D000083722 Bacillus thuringiensis Toxins Endotoxins produced by BACILLUS THURINGIENSIS used in transgenic plants and insecticides. When eaten by a susceptible insect they are protease activated in the insect midgut resulting in death from bacterial septicemia. B thuringiensis Toxins,B. thuringiensis Toxins,Bt Toxin,Bt Toxins,Toxin, Bt,Toxins, B thuringiensis,Toxins, B. thuringiensis
D001413 Bacillus thuringiensis A species of gram-positive bacteria which may be pathogenic for certain insects. It is used for the biological control of the Gypsy moth. Bacilan,Dipel,Thuricide
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D001427 Bacterial Toxins Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases. Bacterial Toxin,Toxins, Bacterial,Toxin, Bacterial
D015202 Protein Engineering Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes. Genetic Engineering of Proteins,Genetic Engineering, Protein,Proteins, Genetic Engineering,Engineering, Protein,Engineering, Protein Genetic,Protein Genetic Engineering

Related Publications

Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
Biochemical characterization of Bacillus thuringiensis cytolytic delta-endotoxins.
August 1994, Microbiology (Reading, England),
Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
Growth, sporulation, delta-endotoxins synthesis, and toxicity during culture of bacillus thuringiensis H14.
August 2005, Current microbiology,
Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
Cross-resistance of Cry1Ab-selected Ostrinia nubilalis (Lepidoptera: Crambidae) to Bacillus thuringiensis delta-endotoxins.
June 2004, Journal of economic entomology,
Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
[Multiple genes of delta-endotoxins from Bacillus thuringiensis subspecies galleriae].
January 1994, Molekuliarnaia biologiia,
Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
Biochemical and molecular characterization of delta-endotoxins in Bacillus thuringiensis.
November 2009, Folia microbiologica,
Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
[The role of succinate dehydrogenase in bacterial resistance to the delta endotoxins of Bacillus thuringiensis].
January 2005, Mikrobiologiia,
Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
Regulation of the packaging of Bacillus thuringiensis delta-endotoxins into inclusions.
November 2001, Applied and environmental microbiology,
Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
Asporogenous Bacillus thuringiensis Mutant Producing High Yields of delta-Endotoxin.
June 1982, Applied and environmental microbiology,
Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
Production of multiple delta-endotoxins by Bacillus thuringiensis: delta-endotoxins produced by strains of the subspecies galleriae and wuhanensis.
December 1994, Canadian journal of microbiology,
Slim Tounsi, and Nabil Zouari, and Dhouha Ghribi, and Anis J'mal, and Samir Jaoua
Efficacy of lepidopteran specific delta-endotoxins of bacillus thuringiensis against helicoverpa armigera
November 1998, Journal of invertebrate pathology,
Copied contents to your clipboard!