Biomaterial Database

Cryptosporidium infection in domestic geese (Anser anser f. domestica) detected by in-situ hybridization. 2006

S Chvala, and K Fragner, and R Hackl, and M Hess, and H Weissenböck

Department of Pathobiology, Institute of Pathology and Forensic Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.

An in-situ hybridization (ISH) procedure was developed for the detection of Cryptosporidium sp. in paraffin wax-embedded tissues with a digoxigenin-labelled probe targeting the 18S rRNA. This technique was used in addition to traditional methods, such as haematoxylin and eosin staining, periodic acid-Schiff reaction, transmission electron microscopy and the polymerase chain reaction, to examine the bursa of Fabricius (BF), conjunctiva and other tissues from 20 domestic geese aged 16-36 days for the presence of cryptosporidia. Positive signals were found to a moderate or marked extent in both conjunctival samples (89%) and BF samples (88%) but not in other tissues. Sequencing of the PCR amplification product revealed identity with Cryptosporidium baileyi. The infected geese showed no clinical signs and only scanty histological lesions. These results confirm reports showing that young waterfowl are especially vulnerable to cryptosporidium infection and indicate that the BF and conjunctiva are the preferred sites for the presence of the protozoon. ISH proved a good method for detecting and identifying even small numbers of cryptosporidia in tissue sections.

UI	MeSH Term	Description	Entries
D010503	Periodic Acid-Schiff Reaction	A histochemical technique for staining carbohydrates. It is based on PERIODIC ACID oxidation of a substance containing adjacent hydroxyl groups. The resulting aldehydes react with Schiff reagent to form a colored product.	PAS Reaction,PAS Reactions,Periodic Acid Schiff Reaction,Periodic Acid-Schiff Reactions,Reaction, PAS,Reaction, Periodic Acid-Schiff,Reactions, PAS,Reactions, Periodic Acid-Schiff
D011201	Poultry Diseases	Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild.	Disease, Poultry,Diseases, Poultry,Poultry Disease
D011529	Protozoan Infections, Animal	Infections with unicellular organisms formerly members of the subkingdom Protozoa. The infections may be experimental or veterinary.	Animal Protozoan Infection,Animal Protozoan Infections,Infection, Animal Protozoan,Infections, Animal Protozoan,Protozoan Infection, Animal
D002060	Bursa of Fabricius	An epithelial outgrowth of the cloaca in birds similar to the thymus in mammals. It atrophies within 6 months after birth and remains as a fibrous remnant in adult birds. It is composed of lymphoid tissue and prior to involution, is the site of B-lymphocyte maturation.	Fabricius Bursa
D003228	Conjunctiva	The mucous membrane that covers the posterior surface of the eyelids and the anterior pericorneal surface of the eyeball.	Bulbar Conjunctiva,Palpebral Conjunctiva,Plica Semilunaris of Conjunctiva,Plicae Semilunares of Conjunctiva,Tunica Conjunctiva,Conjunctiva, Bulbar,Conjunctiva, Palpebral,Conjunctivas
D003457	Cryptosporidiosis	Intestinal infection with organisms of the genus CRYPTOSPORIDIUM. It occurs in both animals and humans. Symptoms include severe DIARRHEA.	Cryptosporidium Infection,Cryptosporidioses,Cryptosporidium Infections,Infection, Cryptosporidium
D003458	Cryptosporidium	A genus of coccidian parasites of the family CRYPTOSPORIDIIDAE, found in the intestinal epithelium of many vertebrates including humans.	Cryptosporidiums
D005777	Geese	Any of various large waterfowl in the order Anseriformes, especially those of the genera Anser (gray geese) and Branta (black geese). They are larger than ducks but smaller than swans, prefer FRESH WATER, and occur primarily in the northern hemisphere.	Goose,Gooses
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012337	RNA, Ribosomal, 18S	Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.	18S Ribosomal RNA,18S RRNA,RNA, 18S Ribosomal,Ribosomal RNA, 18S