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European seroepidemiology network 2: Standardisation of assays for seroepidemiology of varicella zoster virus. 2006
Fernando de Ory, and
José Manuel Echevarría, and
George Kafatos, and
Cleo Anastassopoulou, and
Nick Andrews, and
Josephine Backhouse, and
Guy Berbers, and
Blazena Bruckova, and
Daniel I Cohen, and
Hester de Melker, and
Irja Davidkin, and
Giovanni Gabutti, and
Louise M Hesketh, and
Kari Johansen, and
Sari Jokinen, and
Lindsay Jones, and
Anika Linde, and
Elisabeth Miller, and
Joël Mossong, and
Anthony Nardone, and
Maria Cristina Rota, and
Andreas Sauerbrei, and
François Schneider, and
Zahava Smetana, and
Annedore Tischer, and
Athanassios Tsakris, and
Robert Vranckx
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. fory@isciii.es
BACKGROUND The aim of the European Sero-Epidemiology Network (ESEN2) is to harmonise the serological surveillance of vaccine-preventable diseases in Europe. OBJECTIVE To allow comparison of antibody prevalence in different countries by standardising results into common units. METHODS For varicella zoster virus (VZV), a reference laboratory established a panel of 148 samples, characterised by indirect enzyme-immunoassay (ELISA), indirect immunofluorescence, and complement fixation test. Fifty-seven samples were also studied by the fluorescence antibody to membrane antigen test. The geometric mean of the antibody activity (GMAA) obtained from four ELISA determinations was used to characterise each sample of the panel as positive (GMAA: >100 mIU/ml), equivocal (GMAA: 50-100 mIU/ml) or negative (GMAA: <50 mIU/ml) for antibody to VZV (anti-VZV). Thirteen laboratories, using five different ELISA tests, tested the panel. RESULTS Agreement with the reference laboratory was above 85% in all cases, and the R(2) values obtained from regression analysis of the quantitative results were always higher than 0.87. Finally, the regression equations could be used to convert national values into a common unitage. CONCLUSIONS This study confirmed that results for anti-VZV obtained by different ELISA methods can be converted into common units, enabling the comparison of the seroprevalence profiles obtained in the participant countries.
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