Biomaterial Database

Gastrin releasing peptide, but not pentagastrin, stimulates ACTH and cortisol secretion in conscious dogs. 1991

L D Sander, and R M Thomas

Department of Physiology, Meharry Medical College, Nashville, TN 37208.

Gastrin releasing peptide (GRP) is the mammalian equivalent of bombesin. Bombesin has been shown to stimulate canine ACTH and cortisol secretion in a dose-related manner. In the present study, following a 30-40 min saline infusion, a progressively-increasing, stepwise infusion of GRP (60, 600, 1200 pmol/kg per h) or pentagastrin (PG) (2, 4, 8 micrograms/kg per h) was administered. Each drug dose was infused for 40-45 min and blood samples were drawn at 20-22.5 min intervals. GRP caused significant, dose-dependent increases in plasma ACTH (145 +/- 6%, 242 +/- 49%, 270 +/- 78% of control) and cortisol (158 +/- 16%, 218 +/- 26%, 250 +/- 62% of control). The dose of GRP required for a half-maximal cortisol response was 120 pmol/kg per h, while that for ACTH was 140 pmol/kg per h. PG had no effect on either ACTH or cortisol. While PG is apparently not involved in GRP/bombesin release of ACTH or cortisol, similar mechanisms of action for GRP and bombesin are suggested by their equimolar potencies.

UI	MeSH Term	Description	Entries
D007030	Hypothalamo-Hypophyseal System	A collection of NEURONS, tracts of NERVE FIBERS, endocrine tissue, and blood vessels in the HYPOTHALAMUS and the PITUITARY GLAND. This hypothalamo-hypophyseal portal circulation provides the mechanism for hypothalamic neuroendocrine (HYPOTHALAMIC HORMONES) regulation of pituitary function and the release of various PITUITARY HORMONES into the systemic circulation to maintain HOMEOSTASIS.	Hypothalamic Hypophyseal System,Hypothalamo-Pituitary-Adrenal Axis,Hypophyseal Portal System,Hypothalamic-Pituitary Unit,Hypothalamic Hypophyseal Systems,Hypothalamic Pituitary Unit,Hypothalamo Hypophyseal System,Hypothalamo Pituitary Adrenal Axis,Portal System, Hypophyseal
D007262	Infusions, Intravenous	The long-term (minutes to hours) administration of a fluid into the vein through venipuncture, either by letting the fluid flow by gravity or by pumping it.	Drip Infusions,Intravenous Drip,Intravenous Infusions,Drip Infusion,Drip, Intravenous,Infusion, Drip,Infusion, Intravenous,Infusions, Drip,Intravenous Infusion
D008297	Male		Males
D010418	Pentagastrin	A synthetic pentapeptide that has effects like gastrin when given parenterally. It stimulates the secretion of gastric acid, pepsin, and intrinsic factor, and has been used as a diagnostic aid.	Acignost,Gastrin Pentapeptide,Peptavlon,Pentapeptide, Gastrin
D010455	Peptides	Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS.	Peptide,Polypeptide,Polypeptides
D010913	Pituitary-Adrenal System	The interactions between the anterior pituitary and adrenal glands, in which corticotropin (ACTH) stimulates the adrenal cortex and adrenal cortical hormones suppress the production of corticotropin by the anterior pituitary.	Pituitary Adrenal System,Pituitary-Adrenal Systems,System, Pituitary-Adrenal,Systems, Pituitary-Adrenal
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D003243	Consciousness	Sense of awareness of self and of the environment.	Consciousnesses
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D005260	Female		Females