Long-term cryopreservation of mouse sperm. 2006

Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.

The objective was to determine if mouse sperm can maintain their fertilizing ability after being frozen for >10 y and whether the offspring derived from these sperm had normal fertilizing ability and phenotype. We cryopreserved sperm from six strains of mice (C57BL/6J, DBA/2N, BALB/cA, C3H/HeJ, B6D2F1 and B6C3F1) in a solution containing 18% (w/v) raffinose and 3% (w/v) skim milk, and preserved them in liquid nitrogen for >10 y. To assess the normality and fertilizing ability of these sperms, they were thawed and used for in vitro fertilization of oocytes of the same strains. Fertilization rates for C57BL/6J, DBA/2N, BALB/cA, C3H/HeJ, B6D2F1 and B6C3F1 were 66.4, 92.3, 72.8, 32.9, 60.3 and 53.7%, respectively. Furthermore, 38.3, 15.0, 43.3, 26.1, 38.3 and 16.7% of the embryos transferred to pseudopregnant females developed and produced live offspring that had normal phenotype and fertility.

UI MeSH Term Description Entries
D008098 Litter Size The number of offspring produced at one birth by a viviparous animal. Litter Sizes,Size, Litter,Sizes, Litter
D008297 Male Males
D008815 Mice, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation. Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D004624 Embryo Transfer The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO. Blastocyst Transfer,Tubal Embryo Transfer,Tubal Embryo Stage Transfer,Embryo Transfers,Transfer, Embryo,Transfers, Embryo
D005260 Female Females
D005298 Fertility The capacity to conceive or to induce conception. It may refer to either the male or female. Fecundity,Below Replacement Fertility,Differential Fertility,Fecundability,Fertility Determinants,Fertility Incentives,Fertility Preferences,Fertility, Below Replacement,Marital Fertility,Natural Fertility,Subfecundity,World Fertility Survey,Determinant, Fertility,Determinants, Fertility,Fertility Determinant,Fertility Incentive,Fertility Preference,Fertility Survey, World,Fertility Surveys, World,Fertility, Differential,Fertility, Marital,Fertility, Natural,Preference, Fertility,Preferences, Fertility,Survey, World Fertility,Surveys, World Fertility,World Fertility Surveys
D005307 Fertilization in Vitro An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro. Test-Tube Fertilization,Fertilizations in Vitro,In Vitro Fertilization,Test-Tube Babies,Babies, Test-Tube,Baby, Test-Tube,Fertilization, Test-Tube,Fertilizations, Test-Tube,In Vitro Fertilizations,Test Tube Babies,Test Tube Fertilization,Test-Tube Baby,Test-Tube Fertilizations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
July 2009, Animal reproduction science,
Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
January 2020, JBRA assisted reproduction,
Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
April 2024, Genes,
Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
January 2021, Methods in molecular biology (Clifton, N.J.),
Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
June 2022, Cryobiology,
Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
October 2015, Theriogenology,
Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
January 2018, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology],
Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
May 2019, The world journal of men's health,
Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
November 2017, Cold Spring Harbor protocols,
Takehito Kaneko, and Ayako Yamamura, and Yukie Ide, and Mami Ogi, and Tomoko Yanagita, and Naomi Nakagata
June 1998, Laboratory animal science,
Copied contents to your clipboard!