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A simplified assay for the diagnosis of varicella-zoster virus (VZV) infections based on the polymerase chain reaction (PCR) is described. Omitting the procedures for extraction and purification of DNA, the crude vesicle fluid materials were used for PCR. Moreover, hybridization was not necessary for detection of the amplification products because they were already visible after ethidium bromide staining of the electrophoresis gel. Results could therefore be obtained within one day. In comparison to virus isolation, PCR proved much more rapid, highly sensitive, and specific. DNA extraction and a double PCR assay with nested primers were necessary for detection of latent VZV infections in trigeminal and thoracic ganglia. The data suggest that the procedures described are universally applicable to several types of specimens dependent on the calculated amount of target DNA.
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
February 1993,
Journal of clinical microbiology,
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
January 1996,
Scandinavian journal of infectious diseases. Supplementum,
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
February 1997,
American journal of ophthalmology,
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
July 2023,
Cureus,
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
August 2000,
American journal of respiratory and critical care medicine,
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
April 1993,
Journal of virology,
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
February 1993,
Archives of ophthalmology (Chicago, Ill. : 1960),
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
May 1991,
The Journal of infectious diseases,
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
January 1992,
The Journal of infectious diseases,
D Dlugosch, and
A M Eis-Hübinger, and
J P Kleim, and
R Kaiser, and
E Bierhoff, and
K E Schneweis
November 2007,
International journal of dermatology,
