Biomaterial Database

Rift Valley fever virus noncoding regions of L, M and S segments regulate RNA synthesis. 2006

Nicolas Gauliard, and Agnès Billecocq, and Ramon Flick, and Michèle Bouloy

Institut Pasteur, Unité de Génétique Moléculaire des Bunyaviridés, 25 rue du Dr Roux 75724 Paris cedex 15, France.

Rift Valley fever virus (RVFV) (Phlebovirus, Bunyaviridae) possesses a genome composed of three negative-stranded RNA molecules. Each segment contains 3' and 5' noncoding regions with terminal complementary sequences forming a panhandle structure. We showed that transcription-replication of the L, M and S segments is regulated, and we established a minigenome rescue system expressing a CAT reporter to investigate the role of the noncoding regions in this process. The L, M and S segment-based minigenomes were shown to drive bona fide transcription and replication and to express variable levels of CAT reporter, indicating differential promoter activities within the noncoding sequences. In addition, we found a good correlation between the relative promoter strength and the abundance of viral RNA species in RVFV-infected cells. Altogether, these results show that RVFV minigenomes are powerful tools to study transcription and replication and constitute a valuable basis to rescue infectious virus from cDNAs.

UI	MeSH Term	Description	Entries
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012296	Rift Valley fever virus	A mosquito-borne species of the PHLEBOVIRUS genus found in eastern, central, and southern Africa, producing massive hepatitis, abortion, and death in sheep, goats, cattle, and other animals. It also has caused disease in humans.
D012367	RNA, Viral	Ribonucleic acid that makes up the genetic material of viruses.	Viral RNA
D014158	Transcription, Genetic	The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.	Genetic Transcription
D015967	Gene Expression Regulation, Viral	Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.	Regulation of Gene Expression, Viral,Viral Gene Expression Regulation,Regulation, Gene Expression, Viral
D016679	Genome, Viral	The complete genetic complement contained in a DNA or RNA molecule in a virus.	Viral Genome,Genomes, Viral,Viral Genomes
D021901	DNA, Intergenic	Any of the DNA in between gene-coding DNA, including untranslated regions, 5' and 3' flanking regions, INTRONS, non-functional pseudogenes, and non-functional repetitive sequences. This DNA may or may not encode regulatory functions.	DNA, Junk,DNA, Spacer,Intergenic DNA,Junk DNA,Spacer DNA,Intercistronic Region,Intercistronic Sequence,Intergenic Region,Intergenic Sequence,Sequence, Intergenic,DNAs, Intergenic,DNAs, Junk,DNAs, Spacer,Intercistronic Regions,Intercistronic Sequences,Intergenic DNAs,Intergenic Regions,Intergenic Sequences,Junk DNAs,Region, Intercistronic,Region, Intergenic,Regions, Intercistronic,Regions, Intergenic,Sequence, Intercistronic,Sequences, Intercistronic,Sequences, Intergenic,Spacer DNAs