Factors derived from mouse embryonic stem cells promote self-renewal of goat embryonic stem-like cells. 2006

Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
Shanghai Transgenic Research Center, Shanghai 201203, China.

Goat embryonic stem (ES)-like cells could be isolated from primary materials-inner cell masses (ICMs) and remain undifferentiated for eight passages in a new culture system containing mouse ES cell conditioned medium (ESCCM) and on a feeder layer of mouse embryo fibroblasts (MEFs). However, when cultured in medium without mouse ESCCM, goat ES-like cells could not survive for more than three passages. In addition, no ES-like cells could be obtained when ICMs were cultured on goat embryo fibroblasts or the primary materials-whole goat blastocysts were cultured on MEFs. Goat ES-like cells isolated from ICMs had a normal karyotype and highly expressed alkaline phosphatase. Multiple differentiation potency of the ES-like cells was confirmed by differentiation into neural cells and fibroblast-like cells in vitro. These results suggest that mouse ES cells might secrete factors playing important roles in promoting goat ES-like cells' self-renewal, moreover, the feeder layers and primary materials could also influence the successful isolation of goat ES-like cells.

UI MeSH Term Description Entries
D001755 Blastocyst A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper. Embryo, Preimplantation,Blastocysts,Embryos, Preimplantation,Preimplantation Embryo,Preimplantation Embryos
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D006041 Goats Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP. Capra,Capras,Goat
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013234 Stem Cells Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells. Colony-Forming Units,Mother Cells,Progenitor Cells,Colony-Forming Unit,Cell, Mother,Cell, Progenitor,Cell, Stem,Cells, Mother,Cells, Progenitor,Cells, Stem,Colony Forming Unit,Colony Forming Units,Mother Cell,Progenitor Cell,Stem Cell
D017077 Culture Media, Conditioned Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis). Conditioned Culture Media,Conditioned Culture Medium,Conditioned Media,Conditioned Medium,Culture Medium, Conditioned,Media, Conditioned,Medium, Conditioned
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
February 2006, Stem cells (Dayton, Ohio),
Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
July 2017, Journal of cellular biochemistry,
Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
December 2004, Biology of reproduction,
Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
December 2006, Zhongguo shi yan xue ye xue za zhi,
Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
August 2008, Journal of cell science,
Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
January 2019, Cell & bioscience,
Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
July 2015, Nanoscale,
Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
February 2010, Nature,
Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
December 2007, Stem cells (Dayton, Ohio),
Hai-Bin Tian, and Hong Wang, and Hong-Ying Sha, and Xu-Jun Xu, and Min Zhu, and You-Bing Wu, and Sheng-Hua Cheng, and Jian-Quan Chen, and Yong-Xiang Shi, and Zeng-Liang Bai, and Guo-Xiang Cheng
January 2006, Handbook of experimental pharmacology,
Copied contents to your clipboard!