Biomaterial Database

[Erythrocyte membrane abnormality of essential hypertension with special reference to membrane fluidity and Na(+)-K+ ATPase activity]. 1991

K Sakamoto

Second Department of Internal Medicine, Faculty of Medicine, Kagoshima University.

To clarify the pathogenesis of essential hypertension (EHT), the biophysical property (fluidity) and Na(+)-K+ ATPase activity of the erythrocyte membrane were pursued. Seventeen patients with EHT, 14 normotensives (NT) with familial history of hypertension and 25 healthy controls (CONTROL) without familial history of hypertension were subjected in this study. Electron spin resonance (ESR) method using 5- or 16-stearic acid label (SAL) was used to measure the membrane fluidity of erythrocytes. Since little changes in the Na(+)-K+ ATPase activity may be expected, the bioluminescence method was adopted to measure the enzyme activity. By evaluation of several measuring conditions for the enzyme activity, the method was revealed to be highly sensitive and reproducible, as has been reported by Lechi. A significant decrease was found in the erythrocyte membrane fluidity of EHT patients using 16-SAL with the parameter of peak height ratio (ho/h-1), when compared with that of CONTROL (EHT: 5.31 +/- 0.13; METHODS 5.20 +/- 0.11, p less than 0.05). The decrease of erythrocyte membrane fluidity, values of peak height ratio using 16-SAL, correlated significantly with the systolic blood pressure (r = 0.36 p less than 0.05) and diastolic pressure (r = 0.34 p less than 0.05). The values of peak height ratio of NT (5.26 +/- 0.17) were found in between those of EHT and CONTROL. The levels of erythrocyte membrane Na(+)-K+ ATPase activity in EHT showed higher than those of CONTROL, but the difference was not significant. They did not correlate with the membrane fluidity and the blood pressure. The artificial modifications of membrane cholesterol contents of erythrocytes made the same directional changes in both the membrane fluidity and the enzyme activity. The results showed that the abnormality of biophysical property existed in the deeper portion of the erythrocyte membrane and indicated the possibility of hereditary perpetuation of EHT. As to changes in Na(+)-K+ ATPase activity, no definite contributory relation to EHT has been identified.

UI	MeSH Term	Description	Entries
D006973	Hypertension	Persistently high systemic arterial BLOOD PRESSURE. Based on multiple readings (BLOOD PRESSURE DETERMINATION), hypertension is currently defined as when SYSTOLIC PRESSURE is consistently greater than 140 mm Hg or when DIASTOLIC PRESSURE is consistently 90 mm Hg or more.	Blood Pressure, High,Blood Pressures, High,High Blood Pressure,High Blood Pressures
D008297	Male		Males
D008560	Membrane Fluidity	The motion of phospholipid molecules within the lipid bilayer, dependent on the classes of phospholipids present, their fatty acid composition and degree of unsaturation of the acyl chains, the cholesterol concentration, and temperature.	Bilayer Fluidity,Bilayer Fluidities,Fluidities, Bilayer,Fluidities, Membrane,Fluidity, Bilayer,Fluidity, Membrane,Membrane Fluidities
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D004578	Electron Spin Resonance Spectroscopy	A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.	ENDOR,Electron Nuclear Double Resonance,Electron Paramagnetic Resonance,Paramagnetic Resonance,Electron Spin Resonance,Paramagnetic Resonance, Electron,Resonance, Electron Paramagnetic,Resonance, Electron Spin,Resonance, Paramagnetic
D004910	Erythrocyte Membrane	The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.	Erythrocyte Ghost,Red Cell Cytoskeleton,Red Cell Ghost,Erythrocyte Cytoskeleton,Cytoskeleton, Erythrocyte,Cytoskeleton, Red Cell,Erythrocyte Cytoskeletons,Erythrocyte Ghosts,Erythrocyte Membranes,Ghost, Erythrocyte,Ghost, Red Cell,Membrane, Erythrocyte,Red Cell Cytoskeletons,Red Cell Ghosts
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000254	Sodium-Potassium-Exchanging ATPase	An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.	ATPase, Sodium, Potassium,Adenosinetriphosphatase, Sodium, Potassium,Na(+)-K(+)-Exchanging ATPase,Na(+)-K(+)-Transporting ATPase,Potassium Pump,Sodium Pump,Sodium, Potassium ATPase,Sodium, Potassium Adenosinetriphosphatase,Sodium-Potassium Pump,Adenosine Triphosphatase, Sodium, Potassium,Na(+) K(+)-Transporting ATPase,Sodium, Potassium Adenosine Triphosphatase,ATPase Sodium, Potassium,ATPase, Sodium-Potassium-Exchanging,Adenosinetriphosphatase Sodium, Potassium,Pump, Potassium,Pump, Sodium,Pump, Sodium-Potassium,Sodium Potassium Exchanging ATPase,Sodium Potassium Pump
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults