Determination of 17alpha-methyltestosterone in muscle tissues of tilapia, rainbow trout, and salmon using liquid chromatography-tandem mass spectrometry. 2006

Pak-Sin Chu, and Mayda Lopez, and Stan Serfling, and Charlie Gieseker, and Renate Reimschuessel
Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708, USA. Pak.Chu@FDA.GOV

An analytical method was developed to quantitate and confirm the presence of 17alpha-methyltestosterone in the muscles of tilapia, rainbow trout, and salmon. The method employed two liquid-liquid partitioning steps and two solid-phase extraction columns for sample cleanup. The final extracts were analyzed on an isocratic reverse-phase liquid chromatography-tandem mass spectrometry system with atmospheric-pressure chemical ionization in the positive ion mode. The method was validated at levels from 0.40 to 1.6 ng/g, with MT-d3 used as an internal standard. The accuracy was between 100% and 110%, and coefficients of variation of <10% were obtained for all three fish species. Muscle tissues from dosed fish were also assayed to demonstrate the effectiveness of the method for recovering the parent drug.

UI MeSH Term Description Entries
D008777 Methyltestosterone A synthetic hormone used for androgen replacement therapy and as an hormonal antineoplastic agent (ANTINEOPLASTIC AGENTS, HORMONAL). 17 beta Methyltestosterone,17 beta-Hydroxy-17-methyl-4-androsten-3-one,17 beta-Methyltestosterone,17-Epimethyltestosterone,17-alpha-Methyltestosterone,17alpha-Methyl-Testosterone,17alpha-Methyltestosterone,17beta-Hydroxy-17-methyl-4-androsten-3-one,17beta-Methyltestosterone,Android (Methyltestoterone),Android-10,Android-25,Android-5,Mesteron,Mesterone,Metandren,Methitest,Oreton,Testoviron,Testred,Virilon,17 Epimethyltestosterone,17 alpha Methyltestosterone,17 beta Hydroxy 17 methyl 4 androsten 3 one,17alpha Methyl Testosterone,17alpha Methyltestosterone,17beta Hydroxy 17 methyl 4 androsten 3 one,17beta Methyltestosterone,Android 10,Android 25,Android 5
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012474 Salmon Fish of the genera ONCORHYNCHUS and Salmo in the family SALMONIDAE. They are anadromous game fish, frequenting the coastal waters of both the North Atlantic and Pacific. They are known for their gameness as a sport fish and for the quality of their flesh as a table fish. (Webster, 3d ed). Oncorhynchus gorbuscha,Oncorhynchus nerka,Oncorhynchus tshawytscha,Salmo salmo,Salmon, Chinook,Salmon, Pink,Salmon, Sockeye,Chinook Salmon,Pink Salmon,Sockeye Salmon
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013058 Mass Spectrometry An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass
D017210 Tilapia A freshwater fish used as an experimental organism and for food. This genus of the family Cichlidae (CICHLIDS) inhabits Central and South America (one species extends north into Texas), West Indies, Africa, Madagascar, Syria, and coastal India. Oreochromis mossambicus,Tilapia mossambica,Mozambique Tilapia,Mozambique Tilapias,Tilapia, Mozambique,Tilapias, Mozambique
D017686 Oncorhynchus mykiss A large stout-bodied, sometimes anadromous, TROUT found in still and flowing waters of the Pacific coast from southern California to Alaska. It has a greenish back, a whitish belly, and pink, red, or lavender stripes on the sides, with usually a sprinkling of black dots. It is highly regarded as a sport and food fish. Its former name was Salmo gairdneri. The sea-run rainbow trouts are often called steelheads. Redband trouts refer to interior populations of rainbows. Salmo gairdneri,Steelhead,Trout, Rainbow,Trout, Redband,Rainbow Trout,Redband Trout,Steelheads

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