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Immortalization of cell lines with specific functions is important for examination of organ-specific functions in vitro. We established a kidney tubule cell line (TKC2) exhibiting a specific physiological response to hormone from the primary culture of kidneys of adult transgenic mice harboring temperature-sensitive SV40 large T-antigen gene. TKC2 cells showed temperature-sensitive growth in culture and exhibited characters of distal tubule cells such as dome formation at confluent culture and stimulation of cAMP synthesis by arginine vasopressin. These phenotypes are maintained after long passages in culture and may provide a new experimental system for studying renal physiology.
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
November 1991,
Experimental cell research,
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
January 1994,
Contributions to nephrology,
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
January 2002,
Archives of andrology,
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
December 1996,
Cell structure and function,
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
January 1996,
Digestion,
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
November 1996,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
October 2000,
Cell structure and function,
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
January 2000,
AAPS pharmSci,
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
December 1993,
Experimental cell research,
N Yanai, and
T Satoh, and
S Kyo, and
K Abe, and
M Suzuki, and
M Obinata
January 2002,
Journal of andrology,
