BIOMAT Database Logo BIOMAT Database Logo

The sensitive determination of nucleic acids using a fluorescence-quenching method. 2006

Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
Key Laboratory of Colloid and Interface Chemistry of the Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.

Experiments indicated that nucleic acids can quench the fluorescence of the Eu3+ -2-thenoyltrifluoroacetone (TTA)-1,10-phenanthroline (Phen) system. Based on this, a sensitive method for the determination of nucleic acids was proposed. The experiments indicated that under the optimum conditions, the quenched fluorescence intensity was in proportion to the concentration of nucleic acids in the range 1.0 x 10(-11)-1.0 x 10(-6) g/mL for yeast RNA (yRNA), 5.0 x 10(-11)-5.0 x 10(-7) g/mL for fish sperm (fsDNA) and 1.0 x 10(-10)-1.5 x 10(-6) g/mL for calf thymus DNA (ctDNA). Their detection limits were 3.0 x 10(-12), 4.0 x 10(-12) and 5.0 x 10(-11) g/mL, respectively. Therefore, the proposed method is one of the most sensitive methods available. The interaction between nucleic acids and Eu3+ -TTA-Phen is also discussed.

UI MeSH Term Description Entries
D009696 Nucleic Acids High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages. Nucleic Acid,Acid, Nucleic,Acids, Nucleic
D009942 Organometallic Compounds A class of compounds of the type R-M, where a C atom is joined directly to any other element except H, C, N, O, F, Cl, Br, I, or At. (Grant & Hackh's Chemical Dictionary, 5th ed) Metallo-Organic Compound,Metallo-Organic Compounds,Metalloorganic Compound,Organometallic Compound,Metalloorganic Compounds,Compound, Metallo-Organic,Compound, Metalloorganic,Compound, Organometallic,Compounds, Metallo-Organic,Compounds, Metalloorganic,Compounds, Organometallic,Metallo Organic Compound,Metallo Organic Compounds
D010618 Phenanthrolines Phenanthroline
D005063 Europium An element of the rare earth family of metals. It has the atomic symbol Eu, atomic number 63, and atomic weight 152. Europium is used in the form of its salts as coatings for cathode ray tubes and in the form of its organic derivatives as shift reagents in NMR spectroscopy.
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D012996 Solutions The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed) Solution
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013804 Thenoyltrifluoroacetone Chelating agent and inhibitor of cellular respiration. 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione,TTFA cpd,Trifluorothienylacetone

Related Publications

Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
Sensitive determination of nucleic acids using organic nanoparticle fluorescence probes.
June 2005, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
Fluorescence enhancement method for the determination of nucleic acids using cationic cyanine as a fluorescence probe.
March 2004, The Analyst,
Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
The sensitive determination of nucleic acids using fluorescence enhancement of Eu3+-benzoylacetone-cetyltrimethylammonium bromide-nucleic acid system.
September 2005, Journal of fluorescence,
Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
[Determination of nucleic acids using toluidine blue as a fluorescence probe].
January 2006, Guang pu xue yu guang pu fen xi = Guang pu,
Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
Fluorescence quenching of the epsilon-ADP-- A simple sensitive method for the determination of copper(II).
October 1977, Analytica chimica acta,
Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
Determination of nucleic acids using fluorescence probe sensitized by microemulsion.
January 2006, Journal of AOAC International,
Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
Determination of carbazochrome by fluorescence quenching method.
November 2012, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
Fluorescence quenching method for the determination of carbazochrome sodium sulfonate with aromatic amino acids.
January 2013, Luminescence : the journal of biological and chemical luminescence,
Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
Determination of nucleic acids using calcein-neodymium complex as a fluorescence probe.
December 2001, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
Zhen Jia, and Jinghe Yang, and Xia Wu, and Changxia Sun, and Fei Wang, and Shufang Liu, and Feng Wang
A SERS quenching method for the sensitive determination of insulin.
May 2021, Drug testing and analysis,
Copied contents to your clipboard!