Biomaterial Database

Inhibition of adsorption of oral streptococci to saliva treated hydroxyapatite by chitin derivatives. 1991

H Sano, and T Matsukubo, and K Shibasaki, and H Itoi, and Y Takaesu

Tokyo Dental College.

This study estimated the effects of five chitin derivatives low molecular chitosan (LMCS), ethyleneglycol chitin (PEGT), carboxymethyl chitin (PCMT), sulphated chitosan (PSSS), and phosphorylated chitin (PPPT) on the adsorption of three oral streptococci to saliva-treated hydroxyapatite (S-HA). The adsorption was evaluated by measuring the optical density of the bacterial cell suspensions released from saliva-treated hydroxyapatite by 0.5 N HCl. The adsorption of test strains to S-HA progressively decreased in proportion to each additional volume of PEGT, PPPT, or PSSS. PPPT and PSSS quite effectively inhibited the adsorption of S. mutans onto S-HA, but were less effective against S. sanguis and S. mitis. PPPT, PSSS, and PCMT all markedly promoted the desorption of S. mutans cells pre-adsorbed onto S-HA. Pretreatment of S-HA with PPPT, PSSS, or PCMT significantly decreased the subsequent adsorption of S. mutans and S. mitis. Pretreatment of these cells with PEGT also decreased their adsorption to S-HA. These findings suggest that these chitin derivatives may change the ionic natures of the S-HA and the bacterial cell surface, resulting in a less favorable interaction.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D002686	Chitin	A linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in INSECTS and FUNGI. When deacetylated it is called CHITOSAN.
D003741	Dental Deposits	Accumulations of microflora that lead to pathological plaque and calculus which cause PERIODONTAL DISEASES. It can be considered a type of BIOFILMS. It is subtly distinguished from the protective DENTAL PELLICLE.	Materia Alba,Dental Deposit,Deposit, Dental,Deposits, Dental
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006882	Hydroxyapatites	A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)	Hydroxyapatite Derivatives,Derivatives, Hydroxyapatite
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D001422	Bacterial Adhesion	Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.	Adhesion, Bacterial,Adhesions, Bacterial,Bacterial Adhesions
D012463	Saliva	The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.	Salivas
D013291	Streptococcus	A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
D013295	Streptococcus mutans	A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.