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The protein synthesis in normal and in EMC-virus infected mouse islets of Langerhans was investigated. Mouse large glucagon immunoreactivity was determined by an immunoassay after chromatographic separation. It was characterized as a peptide of 16 000 MW with in intracellular half-life of 35-45 min. The proportional reduction of void volume proteins, large glucagon immunoreactivity and proinsulin synthesis after infection shows, that both alpha- and beta-cells are damaged by the virus. A reduction in the synthesis of the three protein fractions was already found 6 hrs after inoculation of the virus and remained nearly constant for 48 hrs. An almost complete breakdown of protein synthesis occurred 60 to 70 hrs after infection and was paralleled by the first light microscopic changes in the islets. The stimulation of proinsulin synthesis by glucose was preserved for 48 hrs after EMC-virus infection.
K G Petersen, and
P Heilmeyer, and
L Kerp
October 1976,
Diabetologia,
K G Petersen, and
P Heilmeyer, and
L Kerp
July 1970,
The Journal of clinical investigation,
K G Petersen, and
P Heilmeyer, and
L Kerp
July 1979,
Diabetologia,
K G Petersen, and
P Heilmeyer, and
L Kerp
January 1968,
Diabetes,
K G Petersen, and
P Heilmeyer, and
L Kerp
August 1969,
Archives internationales de physiologie et de biochimie,
K G Petersen, and
P Heilmeyer, and
L Kerp
January 1976,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
K G Petersen, and
P Heilmeyer, and
L Kerp
September 1990,
The Biochemical journal,
K G Petersen, and
P Heilmeyer, and
L Kerp
June 1969,
Diabetes,
K G Petersen, and
P Heilmeyer, and
L Kerp
September 1981,
FEBS letters,
K G Petersen, and
P Heilmeyer, and
L Kerp
October 1986,
The Journal of biological chemistry,
