Temperature-pH sensitivity of bovine serum albumin protein-microgels based on cross-linked poly(N-isopropylacrylamide-co-acrylic acid). 2006

Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
School of Materials Engineering, Zhengzhou University, Zhengzhou 450052 Henan, PR China. dxhuo@zzu.edu.cn

Monodispersed poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAM/AAc) microgels with various contents of acrylic acid (AAc) and cross-linker N,N'-methylenebisacrylamide (MBAAm) were synthesized by surfactant-free emulsion polymerization. The microgels exhibited affinity of binding bovine serum albumin (BSA) with increase of AAc contents. The adsorption amount of BSA was strongly dependent on suspension pH. The maximum adsorption was observed at around pH 4.0, while both low pH and basic conditions induced the rather low adsorption. In addition, AFM images showed that the microgel particles underwent bridging aggregation by loading BSA. The resultant BSA-microgel particles became more hydrophobic after loading BSA. Importantly, the adsorption of BSA onto PNIPAM/AAc microgel was found to alter the swelling/deswelling behavior of the resultant microgel suspensions. With the increase of BSA concentration, the volume phase transition temperature of BSA-microgel particles shifted to the lower temperature.

UI MeSH Term Description Entries
D008832 Microchemistry The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
D011108 Polymers Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS). Polymer
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005782 Gels Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000178 Acrylamides Colorless, odorless crystals that are used extensively in research laboratories for the preparation of polyacrylamide gels for electrophoresis and in organic synthesis, and polymerization. Some of its polymers are used in sewage and wastewater treatment, permanent press fabrics, and as soil conditioning agents.
D000179 Acrylates Derivatives of acrylic acid (the structural formula CH2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
September 2013, Ultrasonics sonochemistry,
Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
January 2004, Journal of biomaterials science. Polymer edition,
Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
January 1997, Journal of biochemistry,
Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
September 2004, Journal of colloid and interface science,
Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
June 2015, Langmuir : the ACS journal of surfaces and colloids,
Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
October 2000, Journal of colloid and interface science,
Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
May 2011, Colloids and surfaces. B, Biointerfaces,
Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
May 2017, ACS omega,
Dongxia Huo, and Yana Li, and Qingwen Qian, and Takaomi Kobayashi
January 2006, Langmuir : the ACS journal of surfaces and colloids,
Copied contents to your clipboard!