Biomaterial Database

Determination of lycopene and beta-carotene by high-performance liquid chromatography using sudan I as internal standard. 2006

F Xu, and Q P Yuan, and H R Dong

College of Life Science and Technology, Beijing University of Chemical Technology, P.O. Box 75, Beijing 100029, PR China.

A new method, using sudan I as internal standard to determine the content of lycopene or beta-carotene in samples, was developed. According to UV-vis absorption spectra, sudan I, lycopene and beta-carotene all had absorption peaks at 450 nm. They could be separated absolutely by high-performance liquid chromatography (HPLC) with retention time of 2.7, 6.6 and 10.1 min, respectively. The related equations between sudan I and lycopene or beta-carotene content were obtained and verified by determining the content of lycopene or beta-carotene in Blakeslea trispora cells. The relative error was below 1.4% for determining lycopene content and below 1.9% for beta-carotene. Intra-day variability for lycopene determination was less than 3.4% and for beta-carotene was less than 1.4%. The mean recovery of lycopene or beta-carotene was 96.1 and 97.9%, respectively.

UI	MeSH Term	Description	Entries
D009284	Naphthols	Naphthalene derivatives carrying one or more hydroxyl (-OH) groups at any ring position. They are often used in dyes and pigments, as antioxidants for rubber, fats, and oils, as insecticides, in pharmaceuticals, and in numerous other applications.	Hydroxynaphthalene,Hydroxynaphthalenes,Naphthol
D012015	Reference Standards	A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.	Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002338	Carotenoids	The general name for a group of fat-soluble pigments found in green, yellow, and leafy vegetables, and yellow fruits. They are aliphatic hydrocarbons containing 4 terpene subunits.	Carotenes,Carotenoid,Tetraterpene Derivatives,Tetraterpenes,Carotene,Derivatives, Tetraterpene
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D000077276	Lycopene	A carotenoid and red pigment produced by tomatoes, other red fruits and vegetables, and photosynthetic algae. It is a key intermediate in the biosynthesis of other carotenoids, and has antioxidant, anti-carcinogenic, radioprotective, and anti-inflammatory properties.	All-trans-Lycopene,LYC-O-MATO,Lycopene, (13-cis)-isomer,Lycopene, (7-cis,7'-cis,9-cis,9'-cis)-isomer -,Lycopene, (cis)-isomer,Pro-Lycopene,Prolycopene,All trans Lycopene,LYC O MATO,LYCOMATO,Pro Lycopene
D000465	Algorithms	A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.	Algorithm
D013056	Spectrophotometry, Ultraviolet	Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Ultraviolet Spectrophotometry
D015203	Reproducibility of Results	The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.	Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D019207	beta Carotene	A carotenoid that is a precursor of VITAMIN A. Beta carotene is administered to reduce the severity of photosensitivity reactions in patients with erythropoietic protoporphyria (PORPHYRIA, ERYTHROPOIETIC).	Betacarotene,BellaCarotin,Carotaben,Max-Caro,Provatene,Solatene,Vetoron,beta-Carotene,Carotene, beta,Max Caro,MaxCaro