Biomaterial Database

Reactions of hydrated electrons with horseradish peroxidase: a pulse radiolysis study. 1991

L Gebicka, and J L Gebicki

Institute of Applied Radiation Chemistry, Technical University of Lódź, Poland.

Kinetics of the reactions of hydrated electrons with horseradish peroxidase (HRP) have been studied by pulse radiolysis. Hydrated electrons reduce HRP to its ferrous form with a yield of 40%. At higher dose (250 Gy) the formation of compound I followed by its reduction to compound II was also observed.

UI	MeSH Term	Description	Entries
D011675	Pulse Radiolysis	Use of a pulse of X-rays or fast electrons to generate free radicals for spectroscopic examination.	Radiolysis, Pulse
D004583	Electrons	Stable elementary particles having the smallest known negative charge, present in all elements; also called negatrons. Positively charged electrons are called positrons. The numbers, energies and arrangement of electrons around atomic nuclei determine the chemical identities of elements. Beams of electrons are called CATHODE RAYS.	Fast Electrons,Negatrons,Positrons,Electron,Electron, Fast,Electrons, Fast,Fast Electron,Negatron,Positron
D005609	Free Radicals	Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. Free radicals include reactive oxygen and nitrogen species (RONS). They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.	Free Radical
D006735	Horseradish Peroxidase	An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.	Alpha-Peroxidase,Ferrihorseradish Peroxidase,Horseradish Peroxidase II,Horseradish Peroxidase III,Alpha Peroxidase,II, Horseradish Peroxidase,III, Horseradish Peroxidase,Peroxidase II, Horseradish,Peroxidase III, Horseradish,Peroxidase, Ferrihorseradish,Peroxidase, Horseradish