Simultaneous analysis of alkaloids from Zanthoxylum nitidum by high performance liquid chromatography-diode array detector-electrospray tandem mass spectrometry. 2006

Mingjin Liang, and Weidong Zhang, and Jiang Hu, and Runhui Liu, and Chuan Zhang
School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.

The chemical profiles of nine alkaloids in Zanthoxylum nitidum, including berberubine, coptisine, sanguinarine, nitidine, chelerythrine, liriodenine, 6,7,8-trimethoxy-2,3-methylendioxybenzophenantridine, oxyavicine and dihydrochelerythrine, were identified by using high performance liquid chromatography-diode array detector-electrospray tandem mass spectrometry (HPLC-DAD-ESI-MS), and a novel and sensitive HPLC-UV method had been developed to simultaneously determine these alkaloids in 70% methanol extract of Zanthoxylum nitidum. The chromatographic separation was performed on an Agilent C(18) analytical column (5 microm, 4.6 mm i.d., 250 mm length) with a gradient solvent system of acetonitrile-0.1% formic buffer (adjusted to pH 4.5 with triethylamine). The methodological validation was carried out and the linearities (r(2)>0.9997) and recoveries (ranged from 98.3% to 101.1%) were acceptable. The limits of detection (LOD) of these alkaloids were ranged from 0.6 ng to 1.5 ng. The results indicated that the contents of alkaloids in Zanthoxylum nitidum varied significantly from habitat to habitat with contents ranged from 0.03 mg/g to 3.34 mg/g. The proposed method is simple, effective and suitable for the quality control of this traditional Chinese medicine (TCM). It suggests that it is necessary to control its quality so as to insure efficacy and safety of TCM.

UI MeSH Term Description Entries
D012015 Reference Standards A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy. Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002138 Calibration Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output. Calibrations
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D000470 Alkaloids Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed) Alkaloid,Plant Alkaloid,Plant Alkaloids,Alkaloid, Plant,Alkaloids, Plant
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D015394 Molecular Structure The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds. Structure, Molecular,Molecular Structures,Structures, Molecular
D021241 Spectrometry, Mass, Electrospray Ionization A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry. ESI Mass Spectrometry,Electrospray Ionization Mass Spectrometry,Mass Spectrometry, ESI,Spectrometry, ESI Mass
D032106 Zanthoxylum A plant genus of the family RUTACEAE. Some members of Zanthoxylum are reclassified from ELEUTHEROCOCCUS, Melicope, and EVODIA. The twigs are used as dental brushing sticks (TOOTHBRUSHING). Most plants that are called Fagara have been reclassified as Zanthoxylum, however some Fagara were reclassified to MELICOPE (also in the Rutacea family) or to GLEDITSIA (a genus in the FABACEAE family). Xanthoxylum,Fagara ailanthoides,Fagara boninensis,Fagara coco,Fagara cuspidata,Fagara heitzii,Fagara leprieurii,Fagara macrophylla,Fagara mayu,Fagara monophyllum,Fagara nitidum,Fagara rhetza,Fagara rubescens,Fagara tessmannii,Fagara xanthoxyloides,Fagara zanthoxyloides,Xanthoxylum cuspidatum,Xanthoxylum inerme,Zanthoxylum ailanthoides,Zanthoxylum coco,Zanthoxylum fagara,Zanthoxylum gilletii,Zanthoxylum heitzii,Zanthoxylum leprieurii,Zanthoxylum mayu,Zanthoxylum monophyllum,Zanthoxylum nitidum,Zanthoxylum rhetza,Zanthoxylum rubescens,Fagara ailanthoide,Fagara boninenses,Fagara cocos,Fagara cuspidatas,Fagara heitzius,Fagara leprieurius,Fagara macrophyllas,Fagara mayus,Fagara monophyllums,Fagara nitidums,Fagara rhetzas,Fagara rubescen,Fagara tessmannius,Fagara xanthoxyloide,Fagara zanthoxyloide,Xanthoxylum cuspidatums,Xanthoxylum inermes,Xanthoxylums,Zanthoxylum ailanthoide,Zanthoxylum cocos,Zanthoxylum fagaras,Zanthoxylum gilletius,Zanthoxylum heitzius,Zanthoxylum leprieurius,Zanthoxylum mayus,Zanthoxylum monophyllums,Zanthoxylum nitidums,Zanthoxylum rhetzas,Zanthoxylum rubescen,Zanthoxylums,ailanthoide, Fagara,ailanthoide, Zanthoxylum,ailanthoides, Fagara,ailanthoides, Zanthoxylum,boninenses, Fagara,coco, Fagara,coco, Zanthoxylum,cocos, Fagara,cocos, Zanthoxylum,cuspidata, Fagara,cuspidatas, Fagara,cuspidatum, Xanthoxylum,cuspidatums, Xanthoxylum,fagaras, Zanthoxylum,gilletii, Zanthoxylum,gilletius, Zanthoxylum,heitzii, Fagara,heitzii, Zanthoxylum,heitzius, Fagara,heitzius, Zanthoxylum,inerme, Xanthoxylum,leprieurii, Fagara,leprieurii, Zanthoxylum,leprieurius, Fagara,leprieurius, Zanthoxylum,macrophylla, Fagara,macrophyllas, Fagara,mayu, Fagara,mayu, Zanthoxylum,mayus, Fagara,mayus, Zanthoxylum,monophyllum, Fagara,monophyllum, Zanthoxylum,monophyllums, Fagara,monophyllums, Zanthoxylum,nitidum, Fagara,nitidum, Zanthoxylum,nitidums, Fagara,nitidums, Zanthoxylum,rhetza, Fagara,rhetza, Zanthoxylum,rubescen, Fagara,rubescen, Zanthoxylum,rubescens, Fagara,rubescens, Zanthoxylum,tessmannii, Fagara,tessmannius, Fagara,xanthoxyloides, Fagara,zanthoxyloides, Fagara

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