BIOMAT Database Logo BIOMAT Database Logo

Molecular cloning and structural analysis of mouse gene and pseudogenes for proliferating cell nuclear antigen. 1991

M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan.

We have isolated clones containing the entire mouse proliferating cell nuclear antigen (PCNA) gene of 3890 bp and flanking sequences using a rat PCNA cDNA as a probe. The mouse gene has 6 exons whose sequences and junction points of exons with introns are extensively homologous to the human gene while sizes and nucleotide sequences of introns are much less conserved than exons. By a transient expression assay of chloramphenicol acetyltransferase, the promoter of this gene is localized within 200 bp upstream of the transcription initiation site. We have also isolated two processed pseudogenes. Homology between the first one (psi PCNA-I) and the exons of the PCNA gene was 76.8% in the region so far sequenced. The second one (psi PCNA-II) consists of a region highly homologous to the entire exons of the PCNA gene, and only 9 out of total 1256 bp are different from the corresponding exon sequence of the gene. The 5'-flanking region of the psi PCNA-II did not function as an active promoter. Surveys in various wild and laboratory mice genomes suggest that the psi PCNA-II was generated through the reverse transcription process of the PCNA mRNA about 5 x 10(5) years ago in the domesticus subspecies of Mus musculus, the house mouse. The psi PCNA-II is tentatively mapped in the chromosome 17 of the C57BL mouse.

UI MeSH Term Description Entries
D007438 Introns Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes. Intervening Sequences,Sequences, Intervening,Intervening Sequence,Intron,Sequence, Intervening
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009115 Muridae A family of the order Rodentia containing 250 genera including the two genera Mus (MICE) and Rattus (RATS), from which the laboratory inbred strains are developed. The fifteen subfamilies are SIGMODONTINAE (New World mice and rats), CRICETINAE, Spalacinae, Myospalacinae, Lophiomyinae, ARVICOLINAE, Platacanthomyinae, Nesomyinae, Otomyinae, Rhizomyinae, GERBILLINAE, Dendromurinae, Cricetomyinae, MURINAE (Old World mice and rats), and Hydromyinae. Murids,Murid
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D011544 Pseudogenes Genes bearing close resemblance to known genes at different loci, but rendered non-functional by additions or deletions in structure that prevent normal transcription or translation. When lacking introns and containing a poly-A segment near the downstream end (as a result of reverse copying from processed nuclear RNA into double-stranded DNA), they are called processed genes. Genes, Processed,beta-Tubulin Pseudogene,Gene, Processed,Processed Gene,Processed Genes,Pseudogene,Pseudogene, beta-Tubulin,Pseudogenes, beta-Tubulin,beta Tubulin Pseudogene,beta-Tubulin Pseudogenes
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005091 Exons The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA. Mini-Exon,Exon,Mini Exon,Mini-Exons

Related Publications

M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Molecular cloning, structure and expression of the yeast proliferating cell nuclear antigen gene.
January 1990, Nucleic acids research,
M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Human gene for proliferating cell nuclear antigen has pseudogenes and localizes to chromosome 20.
July 1989, Somatic cell and molecular genetics,
M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Cloning, sequencing, and chromosomal localization of two tandemly arranged human pseudogenes for the proliferating cell nuclear antigen (PCNA).
December 1996, Mammalian genome : official journal of the International Mammalian Genome Society,
M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Molecular cloning of Phaseolus vulgaris cDNA encoding proliferating cell nuclear antigen.
February 2007, Journal of plant physiology,
M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Molecular cloning of cDNA coding for rat proliferating cell nuclear antigen (PCNA)/cyclin.
March 1987, The EMBO journal,
M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
The gene for proliferating cell nuclear antigen (Pcna) maps to mouse chromosome 2.
January 1992, Mammalian genome : official journal of the International Mammalian Genome Society,
M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Structural and Functional Insight into Proliferating Cell Nuclear Antigen.
April 2016, Journal of microbiology and biotechnology,
M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Molecular cloning of a maize cDNA clone encoding a putative proliferating cell nuclear antigen.
January 1995, Biochimica et biophysica acta,
M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Molecular cloning and characterization of proliferating cell nuclear antigen (PCNA) from Chinese shrimp Fenneropenaeus chinensis.
October 2008, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
M Yamaguchi, and Y Hayashi, and F Hirose, and S Matsuoka, and T Moriuchi, and T Shiroishi, and K Moriwaki, and A Matsukage
Gene cloning and polymerase chain reaction with proliferating cell nuclear antigen from Thermococcus kodakaraensis KOD1.
October 2002, Bioscience, biotechnology, and biochemistry,
Copied contents to your clipboard!