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Somatostatin cells in the rat medullary reticular formation (Md) have been studied using peroxidase-antiperoxidase immunocytochemistry. Large fusiform and multipolar somatostatin immunoreactive cells were observed in the ventral subnucleus (MdV) running in a band close to the border with the dorsal subnucleus (MdD). In the same region somatostatin-, serotonin- and enkephalin-immunoreactive fibres occur and double staining revealed that these all contact the somatostatin-immunoreactive cells, with enkephalin making a particularly dense innervation.
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