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In pulmonary sarcoidosis, a chronic granulomatous disorder with different stages of activity, the proliferative capacity of the alveolar mononuclear cells is unknown. To get a closer look at this proliferation we combined pulse labeling (by means of tritium thymidine incorporation and autoradiography) with an immunocytochemical staining assay. This assay revealed on one single slide simultaneously blue CD4+ lymphocytes, brown CD8+ lymphocytes and red macrophages. We were able to show that CD4+ as well as CD8+ lymphocytes were radiolabeled only in the active state of the disease. But macrophage proliferation occurred independently of the activity of the disease. In other words with this combination of techniques, it is possible to differentiate, on a single slide, three subsets of mononuclear cells, in combination with their proliferative behavior.
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
January 2010,
Journal of stem cells & regenerative medicine,
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
June 1993,
The Journal of infectious diseases,
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
March 1992,
American journal of veterinary research,
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
December 1996,
Israel journal of medical sciences,
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
June 1995,
The Histochemical journal,
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
May 1984,
Biulleten' eksperimental'noi biologii i meditsiny,
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
January 1990,
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
February 2021,
iScience,
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
November 1984,
The American review of respiratory disease,
T van Maarsseveen, and
H Eckert, and
J de Groot, and
H Renner, and
R Christ, and
R Hennig, and
K Engelmann, and
R Riesner, and
E Laake
August 1997,
Clinica chimica acta; international journal of clinical chemistry,
