BIOMAT Database Logo BIOMAT Database Logo

RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor. 2006

Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom.

Eukaryotic RNA polymerases are large complexes, 12 subunits of which are structurally or functionally homologous across the three polymerase classes. Each class has a set of specific subunits, likely targets of their cognate transcription factors. We have identified and characterized a human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 (antisense of ERCC1) and as CD3epsilon-associated signal transducer (CAST), and here termed CAST or human Pol I-associated factor of 49 kDa (hPAF49), after mouse orthologue PAF49. We provide evidence for growth-regulated Tyr phosphorylation of CAST/hPAF49, specifically in initiation-competent Pol Ibeta complexes in HeLa cells, at a conserved residue also known to be important for signaling during T-cell activation. CAST/hPAF49 can interact with activator upstream binding factor (UBF) and, weakly, with selectivity factor 1 (SL1) at the rDNA (ribosomal DNA repeat sequence encoding the 18S, 5.8S, and 28S rRNA genes) promoter. CAST/hPAF49-specific antibodies and excess CAST/hPAF49 protein, which have no effect on basal Pol I transcription, inhibit UBF-activated transcription following functional SL1-Pol I-rDNA complex assembly and disrupt the interaction of UBF with CAST/hPAF49, suggesting that interaction of this Pol I-specific subunit with UBF is crucial for activation. Drawing on parallels between mammalian and Saccharomyces cerevisiae Pol I transcription machineries, we advance one model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Pol I-template complex and, thereby, activation of transcription.

UI MeSH Term Description Entries
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012318 RNA Polymerase I A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. The enzyme functions in the nucleolar structure and transcribes DNA into RNA. It has different requirements for cations and salts than RNA polymerase II and III and is not inhibited by alpha-amanitin. DNA-Dependent RNA Polymerase I,RNA Polymerase A,DNA Dependent RNA Polymerase I,Polymerase A, RNA,Polymerase I, RNA
D014443 Tyrosine A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin. L-Tyrosine,Tyrosine, L-isomer,para-Tyrosine,L Tyrosine,Tyrosine, L isomer,para Tyrosine
D015533 Transcriptional Activation Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes. Gene Activation,Genetic Induction,Transactivation,Induction, Genetic,Trans-Activation, Genetic,Transcription Activation,Activation, Gene,Activation, Transcription,Activation, Transcriptional,Genetic Trans-Activation,Trans Activation, Genetic
D047908 Intracellular Signaling Peptides and Proteins Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors. Intracellular Signaling Peptides,Intracellular Signaling Proteins,Peptides, Intracellular Signaling,Proteins, Intracellular Signaling,Signaling Peptides, Intracellular,Signaling Proteins, Intracellular
D021122 Protein Subunits Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly. Protomers,Protein Subunit,Protomer,Subunit, Protein,Subunits, Protein
D035463 Pol1 Transcription Initiation Complex Proteins Factors that form a preinitiation complex at promoters that are specifically transcribed by RNA POLYMERASE I. Pol I Transcription Initiation Complex Proteins,Transcription Factors, TFI,Polymerase 1 Complex Proteins,Polymerase I Complex Proteins,Polymerase I Complex Transcription Factors,Transcription Factors, RNA Polymerase I

Related Publications

Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
The species-specific RNA polymerase I transcription factor SL-1 binds to upstream binding factor.
February 1996, Molecular and cellular biology,
Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
RNA polymerase switch in transcription of yeast rDNA: role of transcription factor UAF (upstream activation factor) in silencing rDNA transcription by RNA polymerase II.
April 1999, Proceedings of the National Academy of Sciences of the United States of America,
Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
The RNA polymerase I transcription factor, upstream binding factor, interacts directly with the TATA box-binding protein.
December 1994, The Journal of biological chemistry,
Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
Molecular Topology of RNA Polymerase I Upstream Activation Factor.
June 2020, Molecular and cellular biology,
Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
Role of the RNA polymerase alpha subunit in transcription activation.
November 1992, Molecular microbiology,
Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae: TBP is required for upstream activation factor-dependent recruitment of core factor.
October 1996, Genes & development,
Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
The HMG box-containing nucleolar transcription factor UBF interacts with a specific subunit of RNA polymerase I.
January 1994, The EMBO journal,
Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
hALP, a novel transcriptional U three protein (t-UTP), activates RNA polymerase I transcription by binding and acetylating the upstream binding factor (UBF).
March 2011, The Journal of biological chemistry,
Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
Modifications of both selectivity factor and upstream binding factor contribute to poliovirus-mediated inhibition of RNA polymerase I transcription.
August 2005, The Journal of general virology,
Kostya I Panov, and Tatiana B Panova, and Olivier Gadal, and Kaori Nishiyama, and Takashi Saito, and Jackie Russell, and Joost C B M Zomerdijk
Limited proteolysis unmasks specific DNA-binding of the murine RNA polymerase I-specific transcription termination factor TTFI.
October 1992, Journal of molecular biology,
Copied contents to your clipboard!