Toxin gene profiling of enterotoxic and emetic Bacillus cereus. 2006

Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
Microbial Ecology Group, Department of Biosciences, WZW, Technische Universität München, Freising, Germany. monika.ehling-schulz@wzw.tum.de

Very different toxins are responsible for the two types of gastrointestinal diseases caused by Bacillus cereus: the diarrhoeal syndrome is linked to nonhemolytic enterotoxin NHE, hemolytic enterotoxin HBL, and cytotoxin K, whereas emesis is caused by the action of the depsipeptide toxin cereulide. The recently identified cereulide synthetase genes permitted development of a molecular assay that targets all toxins known to be involved in food poisoning in a single reaction, using only four different sets of primers. The enterotoxin genes of 49 strains, belonging to different phylogenetic branches of the B. cereus group, were partially sequenced to encompass the molecular diversity of these genes. The sequence alignments illustrated the high molecular polymorphism of B. cereus enterotoxin genes, which is necessary to consider when establishing PCR systems. Primers directed towards the enterotoxin complex genes were located in different CDSs of the corresponding operons to target two toxin genes with one single set of primers. The specificity of the assay was assessed using a panel of B. cereus strains with known toxin profiles and was successfully applied to characterize strains from food and clinical diagnostic labs as well as for the toxin gene profiling of B. cereus isolated from silo tank populations.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D004639 Emetics Agents that cause vomiting. They may act directly on the gastrointestinal tract, bringing about emesis through local irritant effects, or indirectly, through their effects on the chemoreceptor trigger zone in the postremal area near the medulla. Emetic,Emetic Agent,Emetic Drug,Emetic Agents,Emetic Drugs,Emetic Effect,Emetic Effects,Agent, Emetic,Agents, Emetic,Drug, Emetic,Drugs, Emetic,Effect, Emetic,Effects, Emetic
D004768 Enterotoxins Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria. Staphylococcal Enterotoxin,Enterotoxin,Staphylococcal Enterotoxins,Enterotoxin, Staphylococcal,Enterotoxins, Staphylococcal
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D005517 Foodborne Diseases Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning. Food Poisoning,Food-borne Disease,Food-borne Illness,Foodborne Disease,Foodborne Illness,Food-borne Diseases,Food-borne Illnesses,Foodborne Illnesses,Poisoning, Food,Disease, Food-borne,Disease, Foodborne,Food Poisonings,Food borne Disease,Food borne Diseases,Food borne Illness,Food borne Illnesses,Illness, Food-borne,Illness, Foodborne,Illnesses, Foodborne
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001409 Bacillus cereus A species of rod-shaped bacteria that is a common soil saprophyte. Its spores are widespread and multiplication has been observed chiefly in foods. Contamination may lead to food poisoning.
D001427 Bacterial Toxins Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases. Bacterial Toxin,Toxins, Bacterial,Toxin, Bacterial
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
July 2010, Journal of food protection,
Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
May 2008, Letters in applied microbiology,
Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
March 2015, Analytical and bioanalytical chemistry,
Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
November 2000, Letters in applied microbiology,
Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
April 1999, Applied and environmental microbiology,
Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
May 2006, International journal of food microbiology,
Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
February 2002, International journal of food microbiology,
Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
October 2011, International journal of food microbiology,
Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
November 2012, The Journal of veterinary medical science,
Monika Ehling-Schulz, and Marie-Hélène Guinebretiere, and Amanda Monthán, and Odile Berge, and Martina Fricker, and Birgitta Svensson
April 1995, Microbiology (Reading, England),
Copied contents to your clipboard!