A simple and rapid determination of biapenem in plasma by high-performance liquid chromatography. 2006

Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
Department of Clinical Pharmacotherapy, Division of Clinical Pharmacotherapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan. ikeda@hiroshima-u.ac.jp

A simple, rapid and precise HPLC method using ultrafiltration to remove plasma protein was developed to determine biapenem concentrations in human plasma. Plasma was separated by centrifugation at 4 degrees C from blood collected in heparinized vacuum tubes, and biapenem was stabilized by immediate mixing the plasma with 1M 3-morpholinopropanesulfonic acid (MOPS) buffer (pH 7.0) (1:1). Biapenem was detected by ultraviolet absorbance at 300nm with no interfering plasma peak. The calibration curve of biapenem in human plasma was linear from 0.04 to 50microg/mL. The limit of detection was 0.01microg/mL, which was more than 40-fold lower than that of conventional plasma protein precipitation using ammonium sulfate. The assay has been clinically applied to pharmacokinetic studies in patients.

UI MeSH Term Description Entries
D012015 Reference Standards A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy. Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry
D013845 Thienamycins Beta-lactam antibiotics that differ from PENICILLINS in having the thiazolidine sulfur atom replaced by carbon, the sulfur then becoming the first atom in the side chain. They are unstable chemically, but have a very broad antibacterial spectrum. Thienamycin and its more stable derivatives are proposed for use in combinations with enzyme inhibitors. Antibiotics, Thienamycin,Thienamycin Antibiotics
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face

Related Publications

Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
July 2011, Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition,
Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
December 1983, Journal of chromatography,
Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
September 1999, Journal of chromatography. B, Biomedical sciences and applications,
Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
August 1983, Journal of chromatography,
Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
February 1979, Journal of chromatography,
Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
June 1992, Journal of chromatography,
Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
October 1985, Journal of chromatography,
Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
July 1997, Journal of chromatography. B, Biomedical sciences and applications,
Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
January 2015, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Kayo Ikeda, and Kazuro Ikawa, and Aki Ikeda, and Yoshimi Nishikawa, and Norifumi Morikawa
January 1982, Journal of chromatography,
Copied contents to your clipboard!