-transparent.png){kind=logo}
Among various phosphate acceptor proteins and peptides so far tested, a synthetic peptide having the sequence surrounding Ser(8) of myelin basic protein, Gln-Lys-Arg-Pro-Ser(8)-Gln-Arg-Ser-Lys-Tyr-Leu, (MBP4-14), is the most specific and convenient substrate which can be used for selective assay of protein kinase C. This peptide is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca2+/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts. The Km value is considerably low (7 microM) with a Vmax value of twice as much as that for H1 histone.
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
March 1997,
The Biochemical journal,
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
June 1991,
The Kobe journal of medical sciences,
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
June 1989,
FEBS letters,
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
December 1993,
Analytical biochemistry,
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
August 1994,
Analytical biochemistry,
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
January 1983,
Analytical biochemistry,
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
October 1986,
Biochimica et biophysica acta,
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
December 1989,
FEBS letters,
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
February 1985,
Proceedings of the National Academy of Sciences of the United States of America,
I Yasuda, and
A Kishimoto, and
S Tanaka, and
M Tominaga, and
A Sakurai, and
Y Nishizuka
March 1994,
The Journal of biological chemistry,
