BIOMAT Database Logo BIOMAT Database Logo

Identification of Salmonella somatic and flagellar antigens by modified serological methods. 1990

R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
Enteric Bacteriology Laboratory, New York City Department of Health, Bureau of Laboratories, New York 10016.

This report describes two modified methods for the identification of Salmonella somatic (O) and flagellar (H) antigens. Over a period of 2 years, both modified methods were found to be approximately three times less labor intensive than the standard methods while requiring no more technical skill. The modified methods were as accurate as the standard methods in identifying the O and H antigens of 350 Salmonella isolates. Furthermore, 43 O antisera reacted exclusively with organisms possessing homologous O antigens when the modified and two standard methods were used. At the antiserum dilutions used for H antigen identification, H antisera did not react with O antigens or heterologous H antigens by either the modified or the standard method. Compared with the standard method for H antigen identification, the modified method was approximately 20 times more economical with respect to antisera and usually generated a 1.5- to 4-fold higher titer. Since the antisera stored for use in the modified method for H antigen identification were usually 100-fold more dilute than the antisera stored for the standard method, an antibody-stabilizing buffer was incorporated in the diluted antisera, allowing these reagents to be used for at least 9 to 16 months.

UI MeSH Term Description Entries
D007106 Immune Sera Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen. Antisera,Immune Serums,Sera, Immune,Serums, Immune
D011237 Predictive Value of Tests In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test. Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D005407 Flagella A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed) Flagellum
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000372 Agglutination Tests Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed) Agglutination Test,Test, Agglutination,Tests, Agglutination
D000942 Antigens, Bacterial Substances elaborated by bacteria that have antigenic activity. Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D012475 Salmonella A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species

Related Publications

R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
[Fluorescence serological differentiation between somatic and flagellar antigens of Pseudomonas aeruginosa].
January 1977, Zeitschrift fur allgemeine Mikrobiologie,
R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
Serological and finger-printing analyses of mutant flagellar antigens of Salmonella.
December 1970, Journal of general microbiology,
R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
[Complex Salmonella somatic antigens and their identification].
January 1981, Mikrobiyoloji bulteni,
R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
Somatic and flagellar immunofluorescence of Salmonella.
October 1966, Journal of bacteriology,
R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
Identification and phase inversion of Salmonella flagellar antigens, using immuno-discs.
March 2018, The Journal of veterinary medical science,
R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
The response of foetal sheep to the somatic and flagellar antigens of Salmonella typhimurium.
October 1977, The Australian journal of experimental biology and medical science,
R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
[Somatic and flagellar antigens of Yersinia enterocolitica].
May 1971, Annales de l'Institut Pasteur,
R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
Serum antibody to Salmonella flagellar antigens. I. Methods of antibody assay.
December 1968, The Australian journal of experimental biology and medical science,
R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
[ON THE IMMUNOCHEMISTRY OF SOMATIC ANTIGENS OF ENTEROBACTERIACEAE. IX. SEROLOGICAL TYPING OF SALMONELLA R ANTIGENS].
May 1964, Biochemische Zeitschrift,
R Gruenewald, and D P Dixon, and M Brun, and S Yappow, and R Henderson, and J E Douglas, and M H Backer
[Serological studies of Vibrio parahaemolyticus. II. Flagellar antigens].
November 1968, Nihon saikingaku zasshi. Japanese journal of bacteriology,
Copied contents to your clipboard!