BIOMAT Database Logo BIOMAT Database Logo

Comparison of Dolichos biflorus lectin and other lectin-horseradish peroxidase conjugates in staining of cutaneous blood vessels in the hairless mini-pig. 1990

D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.

Angiogenesis is necessary for normal growth, wound healing, and plays a key role in many pathologic processes. A variety of endothelial markers have been used to investigate angiogenesis. Unfortunately, excellent markers for vascular endothelium in human tissues exhibit little or no staining of endothelia in tissues of other animal species, including the pig. We are interested in the hairless Yucatan strain of mini-pig as an animal model for studying cutaneous wound healing because its skin is histologically and functionally very similar to that of man. Hoping to find a specific marker to identify vascular endothelium in the mini-pig, we therefore screened a battery of 11 different lectin-horseradish peroxidase conjugates. Based on specificity and staining intensity, Dolichos biflorus agglutinin (DBA) was chosen from this battery to investigate vascular changes in the healing of cutaneous wounds in the mini-pig. When compared with routine histologic sections stained with hematoxylin and eosin, blood vessels were much easier to identify in sections stained histochemically with DBA. Lectin histochemistry was particularly useful in investigations of early events in angiogenesis during wound healing when newly derived capillary buds and minute blood vessels were obscured in normal histologic sections by an inflammatory cell infiltrate associated with the healing wound. Ultrastructural lectin cytochemistry revealed staining along the luminal surface and the basolateral plasmalemma of endothelial cells. Histochemical staining with DBA promises to provide a useful method for further investigation of angiogenesis and other vascular phenomena in a variety of normal and pathologic processes using the hairless Yucatan strain of mini-pig as the animal model.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009389 Neovascularization, Pathologic A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions. Angiogenesis, Pathologic,Angiogenesis, Pathological,Neovascularization, Pathological,Pathologic Angiogenesis,Pathologic Neovascularization,Pathological Angiogenesis,Pathological Neovascularization
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001706 Biopsy Removal and pathologic examination of specimens from the living body. Biopsies
D012867 Skin The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013556 Swine, Miniature Genetically developed small pigs for use in biomedical research. There are several strains - Yucatan miniature, Sinclair miniature, and Minnesota miniature. Miniature Swine,Minipigs,Miniature Swines,Minipig,Swines, Miniature
D014945 Wound Healing Restoration of integrity to traumatized tissue. Healing, Wound,Healings, Wound,Wound Healings
D037102 Lectins Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition. Animal Lectin,Animal Lectins,Isolectins,Lectin,Isolectin,Lectin, Animal,Lectins, Animal
D037121 Plant Lectins Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms. Lectins, Plant,Phytagglutinin,Plant Agglutinin,Plant Lectin,Agglutinins, Plant,Phytagglutinins,Plant Agglutinins,Agglutinin, Plant,Lectin, Plant

Related Publications

D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
Lectin-binding pattern in extramammary Paget's disease by horseradish peroxidase (HRP)-labeling method--specific staining with Dolichos biflorus agglutinin (DBA).
January 1985, Archives of dermatological research,
D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
Primary structure of the Dolichos biflorus seed lectin.
May 1987, The Journal of biological chemistry,
D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
Staining of blood cell surfaces with a lectin-horseradish peroxidase method.
October 1973, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
In Vivo Biosynthetic Studies of the Dolichos biflorus Seed Lectin.
December 1989, Plant physiology,
D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
NH2-terminal sequences of the subunits of Dolichos biflorus lectin.
October 1977, FEBS letters,
D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
Production of a Lectin in Tissue Cultures of Dolichos biflorus.
March 1985, Plant physiology,
D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
cDNA cloning and in vitro synthesis of the Dolichos biflorus seed lectin.
September 1987, European journal of biochemistry,
D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
The reaction of the Dolichos biflorus lectin with non-human bloods.
January 1976, Forensic science,
D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
Isolation and characterization of a lectin from the roots of Dolichos biflorus.
November 1987, Archives of biochemistry and biophysics,
D Darr, and K M McCormack, and T Manning, and S Dunston, and D C Winston, and B A Schulte, and T Buller, and S R Pinnell
Biosynthesis of DB58 lectin in cell suspension cultures of Dolichos biflorus.
April 1994, Archives of biochemistry and biophysics,
Copied contents to your clipboard!