Biomaterial Database

Identification of connective tissue components in healing rat gingiva. 1990

H J Grevstad

Department of Periodontology, School of Dentistry, University of Bergen, Norway.

The aim of the study was to examine connective tissue fiber organization during healing of an incisional wound in rat molar gingiva. Forty rats 50 days old were divided into 10 experimental groups each comprising four animals. Following interdental incisions, healing periods of 11-20 days, and then 3H-proline injection, the animals were sacrificed and specimens prepared for light microscopy, autoradiography, and transmission electron microscopy. Isotope labeling with 3H-proline occurred over newly formed collagen in the path of incision, while preexisting tissue was unlabeled. Ultrastructurally, preexisting and new tissue were identified by measurement of collagen fibril thickness. The spatial relationship between new and remaining fibrous tissue in the transitional zone suggests that reunion is established by side-by-side alignment of new and severed fibrils.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D012038	Regeneration	The physiological renewal, repair, or replacement of tissue.	Endogenous Regeneration,Regeneration, Endogenous,Regenerations
D003094	Collagen	A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).	Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D003238	Connective Tissue	Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.	Connective Tissues,Tissue, Connective,Tissues, Connective
D005109	Extracellular Matrix	A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.	Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005881	Gingiva	Oral tissue surrounding and attached to TEETH.	Gums,Interdental Papilla,Papilla, Interdental,Gum
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001345	Autoradiography	The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)	Radioautography
D013194	Staining and Labeling	The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.	Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings