Molecular characterization of a G protein alpha-subunit-encoding gene from Mucor circinelloides. 2006

Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
Instituto de Investigación en Biología Experimental, Facultad de Química, Mexico.

Genes encoding the Galpha subunit were cloned from Mucor circinelloides, a zygomycete dimorphic fungus. There are at least four genes that encode for Galpha subunits, gpa1, gpa2, gpa3, and gpa4. The genes gpa1 and gpa3 were isolated and characterized, and their predicted products showed 36%-67% identity with Galpha subunits from diverse fungi. Northern blot analysis of gpa3 showed that it is present in spores and constitutively expressed during mycelium development and during yeast-mycelium and mycelium-yeast transitions. However, during yeast cell growth, decreased levels of mRNA were observed. Sequence analysis of gpa3 cDNA revealed that Gpa3 encodes a polypeptide of 356 amino acids with a calculated molecular mass of 40.8 kDa. The deduced sequence of Gpa3 protein contains all the consensus regions of Galpha subunits of the Galpha(i/o/t) subfamily except the cysteine near the C terminus for potential ADP-ribosylation by pertussis toxin. This cDNA was expressed in Escherichia coli and purified by affinity chromatography. Based on its electrophoretic mobility in SDS-PAGE, the molecular mass of the His6-tagged Gpa3 was 45 kDa. The recombinant protein was recognized by a polyclonal antibody against a fragment of a human Galpha(i/o/t). Furthermore, the recombinant Gpa3 was ADP-ribosylated by activated cholera toxin and [32P]NAD but not by pertussis toxin. These results indicate that in M. circinelloides the Galpha subunit Gpa3 is expressed constitutively during differentiation.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009089 Mucor A genus of zygomycetous fungi of the family Mucoraceae, order Mucorales. It is primarily saprophytic, but may cause MUCORMYCOSIS in man from spores germinating in the lungs. Mucors
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005656 Fungal Proteins Proteins found in any species of fungus. Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal
D017386 Sequence Homology, Amino Acid The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species. Homologous Sequences, Amino Acid,Amino Acid Sequence Homology,Homologs, Amino Acid Sequence,Homologs, Protein Sequence,Homology, Protein Sequence,Protein Sequence Homologs,Protein Sequence Homology,Sequence Homology, Protein,Homolog, Protein Sequence,Homologies, Protein Sequence,Protein Sequence Homolog,Protein Sequence Homologies,Sequence Homolog, Protein,Sequence Homologies, Protein,Sequence Homologs, Protein
D044385 GTP-Binding Protein alpha Subunits The GTPase-containing subunits of heterotrimeric GTP-binding proteins. When dissociated from the heterotrimeric complex these subunits interact with a variety of second messenger systems. Hydrolysis of GTP by the inherent GTPase activity of the subunit causes it to revert to its inactive (heterotrimeric) form. The GTP-Binding protein alpha subunits are grouped into families according to the type of action they have on second messenger systems. G-Protein alpha Subunit,GTP-Binding Protein alpha Subunit,G Protein alpha Subunit,GTP Binding Protein alpha Subunit,GTP Binding Protein alpha Subunits,alpha Subunit, G-Protein
D018076 DNA, Complementary Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe. Complementary DNA,cDNA,cDNA Probes,Probes, cDNA

Related Publications

Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
September 1995, Gene,
Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
May 1990, Proceedings of the National Academy of Sciences of the United States of America,
Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
December 1989, Gene,
Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
November 1989, The Journal of biological chemistry,
Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
March 1995, Plant molecular biology,
Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
April 2005, Mycological research,
Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
October 1990, Journal of molecular biology,
Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
February 2016, Wei sheng wu xue bao = Acta microbiologica Sinica,
Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
July 1994, Infection and immunity,
Victor Meza-Carmen, and Jesús García-Soto, and Laura Ongay-Larios, and Roberto Coria, and Mario Pedraza-Reyes, and José Arnau, and Georgina Reyna-Lopez, and Guadalupe Martínez-Cadena
July 1991, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!