Biomaterial Database

Preparation of cortical brain slices for electrophysiological recording. 2006

Costa M Colbert

Department of Biology and Biochemistry, University of Houston, TX, USA.

Acute brain slices allow electrophysiological and imaging techniques to be applied in vitro to the study of neuronal ion channels, synaptic plasticity, and whole-cell function in juvenile and adult tissue. Ion channel recordings from small dendritic branches and axons in brain slices have demonstrated considerable functional differences in ion channel function within subregions of single cells. These findings have greatly increased our understanding of neuronal computation. This chapter presents methods for obtaining high-quality brain slices developed to aid visualization of small neuronal structures using differential interference contrast microscopy.

UI	MeSH Term	Description	Entries
D010477	Perfusion	Treatment process involving the injection of fluid into an organ or tissue.	Perfusions
D002540	Cerebral Cortex	The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulci. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions.	Allocortex,Archipallium,Cortex Cerebri,Cortical Plate,Paleocortex,Periallocortex,Allocortices,Archipalliums,Cerebral Cortices,Cortex Cerebrus,Cortex, Cerebral,Cortical Plates,Paleocortices,Periallocortices,Plate, Cortical
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012996	Solutions	The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)	Solution
D013048	Specimen Handling	Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.	Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings